College of Animal Sciences and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
Zhongcheng Feed Technology Co., Ltd., Feicheng 271600, China.
Toxins (Basel). 2022 Oct 13;14(10):702. doi: 10.3390/toxins14100702.
Zearalenone (ZEN), also known as the F-2 toxin, is a common contaminant in cereal crops and livestock products. This experiment aimed to reveal the changes in the proteomics of ZEN-induced intestinal damage in weaned piglets by tandem mass spectrometry tags. Sixteen weaned piglets either received a basal diet or a basal diet supplemented with 3.0 mg/kg ZEN in a 32 d study. The results showed that the serum levels of ZEN, α-zearalenol, and β-zearalenol were increased in weaned piglets exposed to ZEN (p < 0.05). Zearalenone exposure reduced apparent nutrient digestibility, increased intestinal permeability, and caused intestinal damage in weaned piglets. Meanwhile, a total of 174 differential proteins (DEPs) were identified between control and ZEN groups, with 60 up-regulated DEPs and 114 down-regulated DEPs (FC > 1.20 or <0.83, p < 0.05). Gene ontology analysis revealed that DEPs were mainly involved in substance transport and metabolism, gene expression, inflammatory, and oxidative stress. The Kyoto Encyclopedia of Genes and Genomes analysis revealed that DEPs were significantly enriched in 25 signaling pathways (p < 0.05), most of which were related to inflammation and amino acid metabolism. Our study provides valuable clues to elucidate the possible mechanism of ZEN-induced intestinal injury.
玉米赤霉烯酮（ZEN），又称 F-2 毒素，是谷物作物和畜产品中常见的污染物。本实验旨在通过串联质谱标签揭示 ZEN 诱导断奶仔猪肠道损伤的蛋白质组学变化。16 头断奶仔猪在 32 天的研究中分别接受基础日粮或基础日粮+3.0mg/kg ZEN 补充日粮。结果表明，暴露于 ZEN 的断奶仔猪血清中 ZEN、α-玉米赤霉烯醇和β-玉米赤霉烯醇水平升高（p<0.05）。ZEN 暴露降低了表观养分消化率，增加了肠道通透性，并导致断奶仔猪肠道损伤。同时，在对照组和 ZEN 组之间鉴定出 174 个差异蛋白（DEPs），其中 60 个上调 DEPs 和 114 个下调 DEPs（FC>1.20 或<0.83，p<0.05）。GO 分析表明，DEPs 主要参与物质转运和代谢、基因表达、炎症和氧化应激。KEGG 分析表明，DEPs 在 25 个信号通路中显著富集（p<0.05），其中大多数与炎症和氨基酸代谢有关。本研究为阐明 ZEN 诱导的肠道损伤的可能机制提供了有价值的线索。
