Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Province), College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China.
Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Chengdu, China.
J Anim Sci. 2024 Jan 3;102. doi: 10.1093/jas/skae208.
Zearalenone (ZEN) is a nonsteroidal estrogenic mycotoxin produced by Fusarium strains that is harmful to the intestinal health of animals and is widely present in contaminated crops. The objective of this study was to investigate the potential therapeutic target of ZEN-induced jejunal damage in weaned gilts. Sixteen weaned gilts either received a basal diet or a basal diet supplemented with 3.0 mg/kg ZEN in a 32-d experiment. The results showed that ZEN at the concentration of 3.0 mg/kg diet activated the inflammatory response and caused oxidative stress of gilts (P < 0.05). ZEN exposure resulted in the upregulation (P < 0.05) of the Exchange protein directly activated by the cAMP 1/Ras-related protein1/c-Jun N-terminal kinase (Epac1/Rap1/JNK) signaling pathway in the jejunum of gilts in vivo and in the intestinal porcine epithelial cells in vitro. The cell viability, EdU-positive cells, and the mRNA expression of B-cell lymphoma-2 (Bcl-2) were decreased, whereas the reactive oxygen species production and the mRNA expressions of Bcl-2-associated X (Bax) and Cysteine-aspartic acid protease 3 (Caspase3) were increased (P < 0.05) by ZEN. However, ZEN increased the mRNA expression of Bcl-2 and decreased the mRNA expressions of Bax and caspase3 (P < 0.05) after the Epac1 was blocked. These results collectively indicated that a 3.0 mg ZEN /kg diet induced jejunal damage via the Epac1/Rap1/JNK signaling pathway.
玉米赤霉烯酮（ZEN）是一种由镰刀菌属菌株产生的非甾体类雌激素毒素，对动物的肠道健康有害，广泛存在于受污染的作物中。本研究旨在探讨 ZEN 诱导断奶仔猪空肠损伤的潜在治疗靶点。在为期 32 天的实验中，16 头断奶仔猪分别接受基础日粮或基础日粮加 3.0mg/kg ZEN 日粮。结果表明，日粮中 ZEN 浓度为 3.0mg/kg 可激活炎症反应，导致仔猪发生氧化应激（P<0.05）。ZEN 暴露导致体内仔猪空肠和体外肠猪上皮细胞中 Exchange protein directly activated by the cAMP 1/Ras-related protein1/c-Jun N-terminal kinase（Epac1/Rap1/JNK）信号通路的上调（P<0.05）。细胞活力、EdU 阳性细胞和 B 细胞淋巴瘤-2（Bcl-2）的 mRNA 表达降低，而活性氧的产生和 Bcl-2 相关 X（Bax）和半胱氨酸天冬氨酸蛋白酶 3（Caspase3）的 mRNA 表达增加（P<0.05）。然而，在阻断 Epac1 后，ZEN 增加了 Bcl-2 的 mRNA 表达，降低了 Bax 和 caspase3 的 mRNA 表达（P<0.05）。这些结果表明，日粮中 3.0mg/kg ZEN 通过 Epac1/Rap1/JNK 信号通路诱导空肠损伤。
