State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
Toxins (Basel). 2022 Oct 17;14(10):710. doi: 10.3390/toxins14100710.
A sensitive dual-readout immunosensor for fluorescence and electrochemiluminescence (ECL) detection of ricin was established, which was combined with a streptavidin-biotin signal amplification system. CdSe/ZnS quantum dots with fine fluorescence and ECL properties were used as the dual-signal function probes of the sandwich immunocomplex. Under the optimum experimental conditions, the dual signal intensity increased significantly with the rise in ricin concentration. The fluorescence intensity of the senor exhibited a good liner relationship toward the ricin concentrations with 0.1~100 ng/mL and the limit of detection (LOD) was 81.7 pg/mL; taking ECL as the detection signal, the sensor showed a linear relationship with the ricin concentrations ranging from 0.01 ng/mL to 100 ng/mL and the LOD was 5.5 pg/mL. The constructed sensor with high sensitivity had been successfully applied to the detection of ricin in complex matrices with satisfactory recoveries. The proposed immunosensor model can be extended to the analysis and detection of others target proteins.
建立了一种用于蓖麻毒素荧光和电致化学发光（ECL）双重读出的灵敏免疫传感器，该传感器结合了链霉亲和素-生物素信号放大系统。具有良好荧光和 ECL 性能的 CdSe/ZnS 量子点被用作夹心免疫复合物的双重信号功能探针。在最佳实验条件下，随着蓖麻毒素浓度的升高，双重信号强度显著增加。该传感器的荧光强度对 0.1~100ng/mL 范围内的蓖麻毒素浓度表现出良好的线性关系，检测限（LOD）为 81.7pg/mL；以 ECL 为检测信号，传感器对 0.01ng/mL 至 100ng/mL 范围内的蓖麻毒素浓度表现出线性关系，检测限（LOD）为 5.5pg/mL。所构建的具有高灵敏度的传感器已成功应用于复杂基质中蓖麻毒素的检测，回收率令人满意。该免疫传感器模型可扩展用于分析和检测其他目标蛋白。
