Sadaow Lakkhana, Rodpai Rutchanee, Janwan Penchom, Boonroumkaew Patcharaporn, Sanpool Oranuch, Thanchomnang Tongjit, Yamasaki Hiroshi, Ittiprasert Wannaporn, Mann Victoria H, Brindley Paul J, Maleewong Wanchai, Intapan Pewpan M
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand.
Trop Med Infect Dis. 2022 Oct 17;7(10):308. doi: 10.3390/tropicalmed7100308.
Chronic human liver fluke infections caused by and can last for decades and cause liver and biliary diseases, including life-threatening pathology prior to cholangiocarcinoma (CCA). CCA generally has a poor prognosis. Serological diagnosis can support parasitological examination in diagnosing disease and screening for the risk of CCA. Here, we present an improved and innovative lateral flow immunochromatographic test (ICT) kit that uses whole-blood samples (WBS) rather than serum to diagnose human opisthorchiasis, which also successfully diagnosed human clonorchiasis. This ICT includes a soluble worm extract of adults and colloidal-gold-labeled conjugates of the IgG antibody to evaluate the diagnostic values with simulated WBS ( 347). Simulated WBS were obtained by the spiking infection sera with red blood cells. The diagnostic sensitivity, specificity, positive and negative predictive values, and accuracy for detecting opisthorchiasis were 95.5%, 87.0%, 80.5%, 97.2%, and 90.1%, respectively. For clonorchiasis, these findings were 85.7%, 87.0%, 53.6%, 97.2%, and 86.8%, respectively. Combined for both diseases, they were 93.2%, 87.0%, 84.0%, 94.6%, and 89.6%, respectively. The ICT kit can possibly replace the ICT platforms for antibody detection in serum samples in field surveys in remote areas where sophisticated equipment is not available.
由华支睾吸虫和肝片形吸虫引起的慢性人体肝吸虫感染可持续数十年，并导致肝脏和胆道疾病，包括胆管癌（CCA）发生前的危及生命的病变。CCA的预后通常较差。血清学诊断可辅助寄生虫学检查来诊断疾病并筛查CCA风险。在此，我们展示了一种改进的创新性侧向流动免疫层析试验（ICT）试剂盒，其使用全血样本（WBS）而非血清来诊断人体华支睾吸虫病，该试剂盒也成功诊断了人体肝吸虫病。这种ICT试剂盒包含成虫的可溶性虫体提取物以及IgG抗体的胶体金标记结合物，用于评估模拟全血样本（n = 347）的诊断价值。模拟全血样本通过将感染血清与红细胞混合获得。检测华支睾吸虫病的诊断敏感性、特异性、阳性和阴性预测值以及准确率分别为95.5%、87.0%、80.5%、97.2%和90.1%。对于肝吸虫病，这些结果分别为85.7%、87.0%、53.6%、97.2%和86.8%。两种疾病合并计算时，这些指标分别为93.2%、87.0%、84.0%、94.6%和89.6%。在没有精密设备的偏远地区进行现场调查时，该ICT试剂盒可能会取代用于检测血清样本中抗体的ICT平台。
