华支睾吸虫 ω 类谷胱甘肽转移酶是肝吸虫病和并殖吸虫病血清学诊断的可靠标志物。

Clonorchis sinensis omega-class glutathione transferases are reliable biomarkers for serodiagnosis of clonorchiasis and opisthorchiasis.

机构信息

Department of Molecular Parasitology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Suwon, South Korea.

Department of Pathology and Tropical Diseases Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

出版信息

Clin Microbiol Infect. 2019 Jan;25(1):109.e1-109.e6. doi: 10.1016/j.cmi.2018.03.042. Epub 2018 Apr 9.

DOI:10.1016/j.cmi.2018.03.042

PMID:29649604

Abstract

OBJECTIVES

To determine the potential for immunodiagnostic application of two recombinant forms of Clonorchis sinensis omega-class glutathione transferases (rCsGSTo1 and rCsGSTo2) against human small liver-fluke C. sinensis and Opisthorchis viverrini infections.

METHODS

Specific antibody levels against rCsGSTo1 and rCsGSTo2 in patients' sera of egg-positive opisthorchiasis (n = 87) and clonorchiasis (n = 120), as well as those in sera from patients with other helminthic infections (n = 252) and healthy controls (n = 40) were retrospectively analysed by ELISA.

RESULTS

We observed highly positive correlation coefficients between specific antibody levels against rCsGSTo1 and rCsGSTo2 and egg counts per gramme of faeces (EPG) of patients with opisthorchiasis (n = 87; r = 0.88 for rCsGSTo1 and r = 0.90 for rCsGSTo2). Sera from opisthorchiasis patients whose EPG counts >100 (n = 43) revealed high antibody titres against both antigens. Patients' sera with low EPG counts (<100, n = 44) also exhibited reliable sensitivities of 93.2% and 97.7% for rCsGSTo1 and rCsGSTo2, respectively. Sera from clonorchiasis patients showed sensitivities of 90% (108/120 samples) and 89.2% (107/120 sera) for rCsGSTo1 and rCsGSTo2. Overall diagnostic sensitivities for liver-fluke infections were 92.3% for rCsGSTo1 (191/207 samples) and 93.2% for rCsGSTo2 (193/207 samples). Specificities were 89.7% (rCsGSTo1) and 97.6% (rCsGSTo2).

CONCLUSIONS

Detection of specific antibody levels against rCsGSTo1 or rCsGSTo2 might be promising for the serodiagnosis of patients infected with these two phylogenetically close carcinogenic liver-flukes.

摘要

目的

确定两种重组华支睾吸虫ω 类谷胱甘肽 S-转移酶（rCsGSTo1 和 rCsGSTo2）对人肝吸虫小柳原虫 C. sinensis 和麝猫后睾吸虫感染的免疫诊断应用潜力。

方法

采用 ELISA 法检测卵阳性华支睾吸虫病（n=87）和肝吸虫病（n=120）患者血清中 rCsGSTo1 和 rCsGSTo2 的特异性抗体水平，以及其他寄生虫感染患者（n=252）和健康对照者（n=40）血清中的特异性抗体水平。

结果

我们观察到 rCsGSTo1 和 rCsGSTo2 特异性抗体水平与华支睾吸虫病患者粪便每克卵数（EPG）之间存在高度正相关系数（n=87；r=0.88 用于 rCsGSTo1，r=0.90 用于 rCsGSTo2）。EPG 计数>100 的华支睾吸虫病患者血清（n=43）对两种抗原均显示出高抗体滴度。EPG 计数较低（<100，n=44）的患者血清对 rCsGSTo1 和 rCsGSTo2 的敏感性也分别可靠地达到 93.2%和 97.7%。肝吸虫病患者血清对 rCsGSTo1 和 rCsGSTo2 的敏感性分别为 90%（108/120 份样本）和 89.2%（107/120 份血清）。肝吸虫感染的总诊断敏感性 rCsGSTo1 为 92.3%（191/207 份样本），rCsGSTo2 为 93.2%（193/207 份样本）。特异性分别为 89.7%（rCsGSTo1）和 97.6%（rCsGSTo2）。