Shao Yiwen, Zheng Chaoqiang, Liu Keke, Xiong Jiyuan, Wang Xiaolong, Han Mingyue, Li Li, Shi Yanling, Lu Jike, Yi Juanjuan
School of Life Sciences, Zhengzhou University, Zhengzhou, China.
Henan Yinfeng Biological Engineering Technology Co., LTD, Zhengzhou, China.
J Food Biochem. 2022 Dec;46(12):e14490. doi: 10.1111/jfbc.14490. Epub 2022 Oct 26.
In this study, the Chinese yam peel polysaccharide (CYPP) was obtained under the extraction conditions optimized by the Response Surface Methodology (RSM). Further biological properties of CYPP-1 purified from CYPP were also determined. The results indicated that the optimum extraction conditions were an extraction temperature of 90.5°C, a liquid-solid ratio of 28.0 ml/g, and an extraction time of 2.94 h, along with a yield of 8.81 ± 1.48%. CYPP-1 was identified as a kind of heteropolysaccharide mostly composed of glucose and galactose (59.4:1.0). The molecular weights were two main parts of 50.5 kDa (54.77%) and 4.4 kDa (21.02%), and the triple-helix conformation was not formed in CYPP-1. Besides, CYPP-1 showed good biological properties including in vitro antioxidant activity and immunomodulatory function on RAW264.7 cells, as well as favorable hypoglycemic effect. Overall, the high-value utilization of CYPP-1 reveals a broad application prospect in the industrial production of functional foods and pharmaceuticals. PRACTICAL APPLICATIONS: Yam peel, which is discarded in large quantities during postharvest processing, results in the production of tremendous by-products and is a great waste of resources. In this study, the yield of water-soluble polysaccharide from yam peel reached 8.81 ± 1.48%. Besides, the purified CYPP-1 exhibited excellent antioxidant activity, favorable immunomodulatory function, and hypoglycemic effect. The high productivity and bioactive effects are both great merits for Chinese yam peel polysaccharide as a promising candidate for foods and medicines industrial production.
在本研究中，通过响应面法（RSM）优化提取条件获得了山药皮多糖（CYPP）。还测定了从CYPP中纯化得到的CYPP-1的进一步生物学特性。结果表明，最佳提取条件为提取温度90.5℃、液固比28.0 ml/g、提取时间2.94 h，产率为8.81±1.48%。CYPP-1被鉴定为一种杂多糖，主要由葡萄糖和半乳糖组成（59.4:1.0）。分子量主要有50.5 kDa（54.77%）和4.4 kDa（21.02%）两个部分，且CYPP-1未形成三螺旋构象。此外，CYPP-1表现出良好的生物学特性，包括体外抗氧化活性、对RAW264.7细胞的免疫调节功能以及良好的降血糖作用。总体而言，CYPP-1的高值利用在功能性食品和药品的工业生产中显示出广阔的应用前景。实际应用：山药皮在采后加工过程中大量被丢弃，产生大量副产品，造成资源的极大浪费。本研究中，山药皮水溶性多糖的产率达到8.81±1.48%。此外，纯化后的CYPP-1表现出优异的抗氧化活性、良好的免疫调节功能和降血糖作用。高产量和生物活性效应都是山药皮多糖作为食品和药品工业生产的有前途候选物的巨大优点。
