Li Qian, Li Wenzhi, Gao Qunyu, Zou Yuxiao
Carbohydrate Lab, School of Food Science and Engineering, South China Univ. of Technology, Guangzhou 510640, P.R. China.
Sericultural & Agri-Food Research Inst. Guangdong Acad. of Agricultural Sciences, Guangzhou 510610, P.R. China.
J Food Sci. 2017 Oct;82(10):2487-2494. doi: 10.1111/1750-3841.13919. Epub 2017 Sep 14.
Three new Chinese yam polysaccharides (namely HSY, huaishanyao in Chinese) were isolated using the methods of boiled water extraction and stepwise ethanolic precipitation, combined with the tangential flow ultrafiltration membrane system. Their molecular weights were determined by high performance gel permeation chromatography. Three type yam polysaccharides in different molecular weight were isolated: HSY-I (>50 kDa), HSY-II (10 to 50 kDa), HSY-III (<10 kDa). The monosaccharide and glycosidic bond links composition were analyzed with GC and Smith degradation. The structure characteristics were further discussed combined with infrared spectrophotometry. Dexamethasone-induced insulin resistance glucose/lipid metabolism diabetic mice model was established to evaluate the hypoglycemic effect of different concentration of HSY and different molecular weights polysaccharide HSY-I, HSY-II, and HSY-III. The results indicated that the HSY polysaccharide mixture, HSY-I and HSY-II had hypoglycemic effect.
Three polysaccharides from Chinese yam tuber were isolated in this study. Their structures were characterized and hypoglycemic effects were evaluated. The result clearly identified the benefits of this plant as a healthy functional food.
采用沸水提取、分步乙醇沉淀法，并结合切向流超滤膜系统，分离得到三种新型山药多糖（即中国的淮山药中的多糖，简称为HSY）。通过高效凝胶渗透色谱法测定其分子量。分离得到三种不同分子量的山药多糖：HSY-I（>50 kDa）、HSY-II（10至50 kDa）、HSY-III（<10 kDa）。采用气相色谱法（GC）和史密斯降解法分析单糖和糖苷键连接组成。结合红外分光光度法进一步探讨其结构特征。建立地塞米松诱导的胰岛素抵抗性葡萄糖/脂质代谢糖尿病小鼠模型，以评估不同浓度的HSY以及不同分子量的多糖HSY-I、HSY-II和HSY-III的降血糖作用。结果表明，HSY多糖混合物、HSY-I和HSY-II具有降血糖作用。
本研究从山药块茎中分离出三种多糖。对其结构进行了表征，并评估了降血糖作用。结果明确证实了这种植物作为健康功能性食品的益处。
