Department of Food Science and Engineering, Moutai Institute, Renhuai 564507, China.
State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China.
Molecules. 2024 May 1;29(9):2085. doi: 10.3390/molecules29092085.
In order to reduce the waste of peel and maximize its utilization, in this study, on the basis of a single-factor experiment and the response surface method, the optimum technological conditions for the extraction of soluble dietary fiber from peel with the compound enzyme method were obtained. The chemical composition, physical and chemical properties, structural characterization and biological activity of the purified soluble dietary fiber (AP-SDF) from the peel were analyzed. We discovered that that the optimum yield was 20.87% under the conditions of cellulase addition 600 U/g, enzymolysis time 100 min, solid-liquid ratio 1:24 g/mL and enzymolysis temperature 51 °C. At the same time, AP-SDF was a porous network structure cellulose type I acidic polysaccharose mainly composed of arabinoxylan (36.03%), galacturonic acid (27.40%) and glucose (19.00%), which possessed the structural characteristic peaks of the infrared spectra of polysaccharides and the average molecular weight (Mw) was 95.52 kDa with good uniformity. In addition, the AP-SDF exhibited high oil-holding capacity (15.11 /), good water-holding capacity and swelling capacity, a certain antioxidant capacity in vitro, hypoglycemic activity in vitro for α-glucosidase inhibition and hypolipidemic activity in vitro for the binding ability of bile acids and cholesterol. These results will provide a theoretical basis for the development of functional products with antioxidant, hypoglycemic and hypolipidemic effects, which have certain application value in related industries.
为了减少果皮浪费并最大限度地利用其资源，本研究在单因素实验和响应面法的基础上，采用复合酶法从果皮中提取可溶性膳食纤维的最佳工艺条件。对果皮中提取的纯化可溶性膳食纤维（AP-SDF）的化学成分、物理化学性质、结构特征和生物活性进行了分析。结果发现，纤维素酶添加量 600 U/g、酶解时间 100 min、固液比 1:24 g/mL、酶解温度 51°C 时，最佳得率为 20.87%。同时，AP-SDF 是一种多孔网络结构的纤维素 I 型酸性多糖，主要由阿拉伯木聚糖（36.03%）、半乳糖醛酸（27.40%）和葡萄糖（19.00%）组成，具有多糖红外光谱的结构特征峰和平均分子量（Mw）为 95.52 kDa 的良好均一性。此外，AP-SDF 具有较高的持油能力（15.11 /）、良好的持水能力和膨胀能力、一定的体外抗氧化能力、体外对α-葡萄糖苷酶抑制的降血糖活性和体外对胆汁酸和胆固醇结合能力的降血脂活性。这些结果将为开发具有抗氧化、降血糖和降血脂作用的功能产品提供理论依据，在相关行业具有一定的应用价值。
