Collage of Horticulture, China Agricultural University, 100193, Beijing, China.
Commun Biol. 2022 Oct 26;5(1):1134. doi: 10.1038/s42003-022-04010-7.
As global warming intensifies, heat stress has become a major environmental constraint threatening crop production and quality worldwide. Here, we characterize Heat-induced long intergenic noncoding RNA 1 (HILinc1), a cytoplasm-enriched lincRNA that plays a key role in thermotolerance regulation of pear (Pyrus spp.). HILinc1 Target 1 (PbHILT1) which is the target transcript of HILinc1, was stabilized via complementary base pairing to upregulate its expression. PbHILT1 could bind to Heat shock transcription factor A1b (PbHSFA1b) to enhance its transcriptional activity, leading to the upregulation of a major downstream transcriptional regulator, Multiprotein bridging factor 1c (PbMBF1c), during heat response. Transient overexpressing of either HILinc1 or PbHILT1 increases thermotolerance in pear, while transient silencing of HILinc1 or PbHILT1 makes pear plants more heat sensitive. These findings provide evidences for a new regulatory mechanism by which HILinc1 facilitates PbHSFA1b activity and enhances pear thermotolerance through stabilizing PbHILT1 transcripts.
随着全球变暖的加剧，热应激已成为威胁全球作物生产和质量的主要环境限制因素。在这里，我们描述了 Heat-induced long intergenic noncoding RNA 1（HILinc1），这是一种富含细胞质的 lincRNA，在梨（Pyrus spp.）的耐热性调节中发挥关键作用。HILinc1 的靶标转录本 PbHILT1 通过互补碱基配对稳定，从而上调其表达。PbHILT1 可以与 Heat shock transcription factor A1b（PbHSFA1b）结合，增强其转录活性，从而在热响应过程中上调主要的下游转录调控因子 Multiprotein bridging factor 1c（PbMBF1c）。瞬时过表达 HILinc1 或 PbHILT1 均可提高梨的耐热性，而瞬时沉默 HILinc1 或 PbHILT1 会使梨植株对热更加敏感。这些发现为 HILinc1 通过稳定 PbHILT1 转录本促进 PbHSFA1b 活性和增强梨耐热性的新调控机制提供了证据。
