Yang Yuekun, Lv Shamei, Huang Xiaosan, He Ying, Zhang Xiaoyan, Liu Yu, Wang Guoping, Hong Ni, Wang Liping
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.
Hortic Res. 2025 Jan 3;12(4):uhae359. doi: 10.1093/hr/uhae359. eCollection 2025 Apr.
Pear ring rot disease () is a significant threat to the healthy development of the pear industry. Recent research has identified the functional role of long non-coding RNAs (lncRNAs) in various biological processes of plants. The role of lncRNAs in the pear defense response remains unknown. In this study, transcriptome sequencing was used to analyze lncRNAs in pear stem infected with . It identified 3555 lncRNAs, of which 286 were significantly differentially expressed. GO and KEGG analyses showed that - and -regulated target genes were enriched in multiple disease resistance-related pathways. More specifically, MSTRG.32189, predicted as an endogenous target mimic (eTM), was significantly down-regulated in response to infection, and was confirmed to inhibit the cleavage effect of PcmiR399b on OE-MSTRG.32189 transgenic exhibited lower Pi content and weaker disease resistance to compared with wild type. In pear callus, overexpression of MSTRG.32189 negatively regulated PcmiR399b, which decreased Pi content and reduced disease resistance. Overexpressing PcmiR399b in pear callus exhibited the opposite effects compared with OE-MSTRG.32189. Overexpression and knockout of further clarified that negatively regulates Pi content and disease resistance to infection. Furthermore, the ROS levels and expressions of disease resistance pathway-related genes were regulated by the MSTRG.32189-PcmiR399b- module in transgenic pear callus, which contributed to disease resistance. Overall, our results demonstrated the role of lncRNAs in the pear defense response, revealing that the MSTRG.32189-PcmiR399b- module regulates phosphate accumulation and disease resistance to infection in pear.
梨轮纹病（）对梨产业的健康发展构成重大威胁。最近的研究已经确定了长链非编码RNA（lncRNAs）在植物各种生物学过程中的功能作用。lncRNAs在梨防御反应中的作用仍然未知。在本研究中，利用转录组测序分析感染的梨茎中的lncRNAs。共鉴定出3555个lncRNAs，其中286个有显著差异表达。基因本体（GO）和京都基因与基因组百科全书（KEGG）分析表明，和调控的靶基因富集于多个抗病相关途径。更具体地说，预测为内源性靶标模拟物（eTM）的MSTRG.32189在响应感染时显著下调，并证实其抑制了PcmiR399b对的切割作用。与野生型相比，OE-MSTRG.32189转基因植株表现出较低的磷含量和较弱的抗病性。在梨愈伤组织中，MSTRG.32189的过表达负调控PcmiR399b，降低了磷含量并降低了抗病性。与OE-MSTRG.32189相比，在梨愈伤组织中过表达PcmiR399b表现出相反的效果。的过表达和敲除进一步阐明了负调控磷含量和对感染的抗病性。此外，转基因梨愈伤组织中MSTRG.32189-PcmiR399b-模块调节了活性氧（ROS）水平和抗病途径相关基因的表达，这有助于抗病性。总体而言，我们的结果证明了lncRNAs在梨防御反应中的作用，揭示了MSTRG.32189-PcmiR399b-模块调节梨中磷的积累和对感染的抗病性。
