Chen Chih-Chieh, Chou Yung-Che, Hsu Chia-Yi, Tsai Eing-Mei, Er Tze-Kiong
Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
Division of Laboratory Medicine, Asia University Hospital, Asia University, Taichung 413, Taiwan.
Biomedicines. 2022 Sep 29;10(10):2432. doi: 10.3390/biomedicines10102432.
Endometriosis is a common gynecological disease that affects approximately 5-10% of reproductive-aged women. However, the etiology and pathophysiology of endometriosis are currently unclear. The objective of this study was to identify a potential pathogenic gene of endometriosis using RNA sequencing (RNA-seq) analysis. Human endometrial stromal cells were isolated from four patients receiving surgical treatment for endometriosis during laparoscopic surgery, and RNA-seq was used to examine differentially expressed genes (DEGs) in eutopic and ectopic endometrial stromal cells. The functional significance of the differentially expressed genes was analyzed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. A total of 1309 upregulated and 663 downregulated genes were identified through the analysis of the transcriptomes of eutopic and ectopic endometrial stromal cells. Furthermore, KEGG analysis indicated that these DEGs were mainly enriched in the PI3K-Akt signaling pathway, cytokine-cytokine receptor interaction, and MAPK signaling pathway. Our study identified differential gene expression in eutopic as compared to ectopic endometrial tissue stromal cells. We strongly believe that our findings can bring new insights into the underlying mechanisms of endometriosis. However, future research is necessary to clarify the roles of the identified genes.
子宫内膜异位症是一种常见的妇科疾病，影响着约5%-10%的育龄妇女。然而，子宫内膜异位症的病因和病理生理学目前尚不清楚。本研究的目的是通过RNA测序（RNA-seq）分析确定子宫内膜异位症的一个潜在致病基因。从四名接受腹腔镜手术治疗子宫内膜异位症的患者中分离出人子宫内膜基质细胞，并使用RNA-seq检测在位和异位子宫内膜基质细胞中的差异表达基因（DEG）。使用基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路富集分析来分析差异表达基因的功能意义。通过对在位和异位子宫内膜基质细胞转录组的分析，共鉴定出1309个上调基因和663个下调基因。此外，KEGG分析表明，这些DEG主要富集在PI3K-Akt信号通路、细胞因子-细胞因子受体相互作用和MAPK信号通路中。我们的研究确定了在位子宫内膜组织基质细胞与异位子宫内膜组织基质细胞之间的差异基因表达。我们坚信，我们的发现能够为子宫内膜异位症的潜在机制带来新的见解。然而，有必要进行进一步的研究以阐明所鉴定基因的作用。
