Mohamed Mohamed-Yousif Ibrahim, Abu Jalila, Zakaria Zunita, Khan Abdul Rashid, Abdul Aziz Saleha, Bitrus Asinamai Athliamai, Habib Ihab
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Veterinary Public Health Research Laboratory, Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain P.O. Box 1555, United Arab Emirates.
Antibiotics (Basel). 2022 Sep 20;11(10):1275. doi: 10.3390/antibiotics11101275.
Transmission of pathogenic microorganisms in the last decades has been considered a significant health hazard and pathogenic , particularly antibiotic-resistant strains, have long been identified as a zoonotic problem. This study aimed to investigate multidrug resistant pathogenic isolates from wild birds, chickens, and environment in selected Orang Asli and Malay villages in Peninsular Malaysia. The bacteriological culture-based technique, disc diffusion method, and multiplex Polymerase Chain Reaction (mPCR) assay was used to determine the occurrence of pathogenic strains in the several samples in the study. isolates showed a variety of multi-drug resistant (MDR) antibiotypes and Enteropathogenic (EPEC) and Enteroinvasive (EIEC) were the most predominantly identified pathogenic strains. The findings of this study demonstrated the significance of animal reservoirs and the environment as sources of pathogenic resistant bacteria, and resistance genes. Hence, there is a need for adoption of a practical surveillance approach on MDR pathogens to control foodborne contamination.
在过去几十年中，致病微生物的传播一直被视为重大的健康危害，并且病原体，特别是耐抗生素菌株，长期以来一直被认定为一个人畜共患病问题。本研究旨在调查马来西亚半岛选定的原住民和马来村庄中野生鸟类、鸡以及环境中的多重耐药致病分离株。基于细菌培养的技术、纸片扩散法和多重聚合酶链反应（mPCR）分析被用于确定研究中多个样本中致病菌株的存在情况。分离株显示出多种多重耐药（MDR）抗菌型，并且肠致病性大肠杆菌（EPEC）和肠侵袭性大肠杆菌（EIEC）是最主要鉴定出的致病菌株。本研究结果证明了动物宿主和环境作为致病耐药细菌及耐药基因来源的重要性。因此，有必要采用一种针对多重耐药病原体的实际监测方法来控制食源性污染。
