Wu Tingting, Ma Xiaoxiao, Wang Fengfeng, Xie Linhong, Lv Qingbo, Zeng Minhao, Xu Yu, Qin Siyuan, Chang Qiaocheng
School of Public Health, Shantou University, Shantou 515063, China.
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Animals (Basel). 2022 Oct 20;12(20):2854. doi: 10.3390/ani12202854.
The Onchocercidae family is composed of more than 30 valid nematode species with notable zoonotic potential. Current limitations in molecular characterization methods and species identification are the main obstacles to a better understanding of the biology of Onchocercidae species, particularly in wildlife. This study describes for the first time the complete mitochondrial (mt) genome sequence of sp. isolated from a wild bird () and belonging to the genus (Nematoda: Onchocercidae). The mt genome of sp. (GenBank accession number: ON641583) was a typical circular DNA molecule of 13,628 bp in size with an AT content of 76.69%. The complete mt genome comprised 36 functional subunits, including 12 protein-coding genes (PCGs), 2 ribosomal RNA genes, and 22 transfer RNA genes. The most common start codon was ATT/ATG except for 2 with TTG, and TAA was the termination codon for all protein-coding genes (PCGs). Phylogenetic analysis of the concatenated and aligned amino acid sequences of the 12 PCGs showed that the trees generated using different methods (Bayesian inference and maximum likelihood) with different partition schemes shared similar topologies. The isolated sp. was placed in the Onchocercidae family and formed a sister branch with the genera and . The entire mt genome of sp. presented in this study could provide useful data for studying the population genetics and phylogenetic relationships of Onchocercidae species.
盘尾丝虫科由30多种有效的线虫物种组成，具有显著的人畜共患病潜力。目前分子表征方法和物种鉴定方面的局限性是更好地了解盘尾丝虫科物种生物学的主要障碍，尤其是在野生动物方面。本研究首次描述了从一只野生鸟类（）分离出的属于盘尾丝虫属（线虫纲：盘尾丝虫科）的某物种的完整线粒体（mt）基因组序列。该物种的mt基因组（GenBank登录号：ON641583）是一个典型的环状DNA分子，大小为13,628 bp，AT含量为76.69%。完整的mt基因组由36个功能亚基组成，包括12个蛋白质编码基因（PCG）、2个核糖体RNA基因和22个转运RNA基因。除了2个以TTG为起始密码子外，最常见的起始密码子是ATT/ATG，所有蛋白质编码基因（PCG）的终止密码子都是TAA。对12个PCG的串联和比对氨基酸序列进行系统发育分析表明，使用不同方法（贝叶斯推断和最大似然法）和不同分区方案生成的树具有相似的拓扑结构。分离出的该物种被置于盘尾丝虫科，并与某属和某属形成一个姐妹分支。本研究中呈现的该物种完整mt基因组可为研究盘尾丝虫科物种的群体遗传学和系统发育关系提供有用数据。
