Min Byeng Ryel, Willis Will, Casey Kenneth, Castleberry Lana, Waldrip Heidi, Parker David
Conservation and Production Research Laboratory, United States Department of Agriculture (USDA)/Agricultural Research Service (ARS), Bushland, TX 79012, USA.
Department of Agricultural and Environmental Sciences, Tuskegee University, Tuskegee, AL 36088, USA.
Animals (Basel). 2022 Oct 21;12(20):2876. doi: 10.3390/ani12202876.
The objectives of this study were to (1) examine the effects of plant condensed (CT) and hydrolyzable tannin (HT) extracts on CH4 and N2O emissions; (2) identify the reactions responsible for manure-derived GHG emissions, and (3) examine accompanying microbial community changes in fresh dairy manure. Five treatments were applied in triplicate to the freshly collected dairy manure, including 4% CT, 8% CT, 4% HT, 8% HT (V/V), and control (no tannin addition). Fresh dairy manure was placed into 710 mL glass incubation chambers. In vitro composted dairy manure samples were collected at 0, 24, 48, and 336 h after the start of incubation. Fluxes of N2O and CH4 were measured for 5-min/h for 14 d at a constant ambient incubation temperature of 39 °C. The addition of quebracho CT significantly decreased the CH4 flux rates compared to the tannin-free controls (215.9 mg/m2/h), with peaks of 75.6 and 89.6 mg/m2/h for 4 and 8% CT inclusion rates, respectively. Furthermore, CT significantly reduced cumulative CH4 emission by 68.2 and 57.3% at 4 and 8% CT addition, respectively. The HT treatments failed to affect CH4 reduction. However, both CT and HT reduced (p < 0.001) cumulative and flux rates of N2O emissions. The decrease in CH4 flux with CT was associated with a reduction in the abundance of Bacteroidetes and Proteobacteria.
（1）研究植物缩合单宁（CT）和水解单宁（HT）提取物对CH4和N2O排放的影响；（2）确定导致源自粪便的温室气体排放的反应，以及（3）研究新鲜奶牛粪便中伴随的微生物群落变化。对新鲜采集的奶牛粪便进行了5种处理，每种处理重复3次，包括4% CT、8% CT、4% HT、8% HT（体积/体积）和对照（不添加单宁）。将新鲜奶牛粪便放入710 mL玻璃培养箱中。在培养开始后的0、24、48和336 h收集体外堆肥的奶牛粪便样本。在39℃的恒定环境培养温度下，连续1天每小时测量5分钟的N2O和CH4通量。与不含单宁的对照（215.9 mg/m2/h）相比，添加坚木CT显著降低了CH4通量率，4%和8% CT添加率时的峰值分别为75.6和89.6 mg/m2/h。此外，添加4%和8% CT时，CT分别显著降低了68.2%和57.3%的CH4累积排放量。HT处理未能影响CH4减排效果，但CT和HT均降低了（p < 0.001）N2O排放的累积量和通量率。CT导致的CH4通量降低与拟杆菌门和变形菌门丰度的降低有关。
