Liu Guang-Hui, Zhang Dian-Guang, Lei Xi-Jun, Tan Xiao-Ying, Song Chang-Chun, Zheng Hua, Luo Zhi
Hubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Antioxidants (Basel). 2022 Sep 26;11(10):1904. doi: 10.3390/antiox11101904.
Currently, the effect of selenium and oxidized fish oil interactions on the intestinal lipid metabolism and antioxidant responses of fish remains unknown. Herein, yellow catfish (weight: 3.99 ± 0.01 g) were used as experimental animals and were fed four diets: an adequate amount of selenium (0.25 mg kg) with fresh fish oil (A-Se+FFO), an adequate amount of selenium with oxidized fish oil (A-Se+OFO), a high amount of selenium (0.50 mg kg) with fresh fish oil (H-Se+FFO), and a high amount of selenium with oxidized fish oil (H-Se+OFO). The feeding experiment was conducted for 10 weeks. The results showed that selenium supplementation alleviated the intestinal tissue damage and reduced the lipid accumulation that was induced by oxidized fish oils. Meanwhile, we also found that 0.50 mg kg selenium reduced the oxidative stress that is caused by oxidized fish oils through increasing the GSH and the activity and mRNA expression of antioxidant enzymes. Dietary selenium and oxidized fish oils also affected the mRNA expression of intestinal selenoproteins including , , and . Mechanistically, Se and oxidized eicosapentaenoic acid (oxEPA) influenced the GSH content by affecting the DNA binding ability of activating transcription factor (ATF) 3 to the promoter. For the first time, our results suggested that selenium alleviated the oxidized fish oil-induced intestinal lipid deposition and the oxidative stress of the fish. We also elucidated the novel mechanism of selenium increasing the GSH content by affecting the interaction of ATF3 and the promoter.
目前，硒与氧化鱼油的相互作用对鱼类肠道脂质代谢和抗氧化反应的影响尚不清楚。在此，以黄颡鱼（体重：3.99±0.01 g）作为实验动物，投喂四种饲料：适量硒（0.25 mg/kg）搭配新鲜鱼油（A-Se+FFO）、适量硒搭配氧化鱼油（A-Se+OFO）、高量硒（0.50 mg/kg）搭配新鲜鱼油（H-Se+FFO）以及高量硒搭配氧化鱼油（H-Se+OFO）。饲养实验进行了10周。结果表明，补充硒可减轻肠道组织损伤，并减少氧化鱼油诱导的脂质积累。同时，我们还发现0.50 mg/kg的硒通过增加谷胱甘肽（GSH）以及抗氧化酶的活性和mRNA表达，降低了氧化鱼油引起的氧化应激。日粮中的硒和氧化鱼油还影响肠道硒蛋白包括、和的mRNA表达。从机制上讲，硒和氧化二十碳五烯酸（oxEPA）通过影响激活转录因子（ATF）3与启动子的DNA结合能力来影响GSH含量。我们的结果首次表明，硒减轻了氧化鱼油诱导的鱼类肠道脂质沉积和氧化应激。我们还阐明了硒通过影响ATF3与启动子的相互作用来增加GSH含量的新机制。
