Ceylanlı Deniz, Şehirli Ahmet Özer, Gençosman Sevgi, Teralı Kerem, Şah Hüseyin, Gülmez Nurhayat, Sayıner Serkan
Department of Biochemistry, Faculty of Veterinary Medicine, Near East University, 99138 Nicosia, North Cyprus, Turkey.
Department of Pharmacology, Faculty of Dentistry, Near East University, 99138 Nicosia, North Cyprus, Turkey.
Antioxidants (Basel). 2022 Sep 28;11(10):1930. doi: 10.3390/antiox11101930.
Alpha-lipoic acid (ALA) is extensively utilized in multivitamin formulas and anti-aging products. The purpose of this study was to investigate the potential protective benefits of ALA on 5-fluorouracil (5-FU)-induced gastrointestinal mucositis in Wistar albino rats. Tissues from the stomach, small intestine, and large intestine were excised, and blood sera were obtained to identify biochemical indices such as TNF-α, IL-1β, MDA, GPx, SOD, MMP-1, -2, -8, and TIMP-1. A histopathological study was also performed. The results revealed mucositis-elevated TNF-, IL-1, MDA, MMP-1, -2, -8, and TIMP-1 levels in both tissues and sera, and these values dropped dramatically following ALA treatment. Reduced SOD and GPx activities in mucositis groups were reversed in ALA-treated groups. The damage produced by mucositis in the stomach and small intestine regressed in the ALA-treated group, according to histopathological evaluation. Consequently, the implementation of ALA supplementation in 5-FU therapy may act as a protective intervention for cancer patients with gastrointestinal mucositis. In light of the findings, ALA, a food-derived antioxidant with pleiotropic properties, may be an effective treatment for 5-FU-induced gastrointestinal mucositus, and prevent oxidative stress, inflammation, and tissue damage in cancer patients receiving 5-FU therapy.
α-硫辛酸（ALA）广泛应用于多种维生素配方和抗衰老产品中。本研究的目的是探讨ALA对5-氟尿嘧啶（5-FU）诱导的Wistar白化大鼠胃肠道黏膜炎的潜在保护作用。切除胃、小肠和大肠组织，并采集血清以确定生化指标，如肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）、基质金属蛋白酶-1（MMP-1）、-2、-8和组织金属蛋白酶抑制剂-1（TIMP-1）。还进行了组织病理学研究。结果显示，黏膜炎使组织和血清中的TNF、IL-1、MDA、MMP-1、-2、-8和TIMP-1水平升高，而ALA治疗后这些值显著下降。黏膜炎组中降低的SOD和GPx活性在ALA治疗组中得到逆转。根据组织病理学评估，ALA治疗组中胃和小肠黏膜炎所产生的损伤有所减轻。因此，在5-FU治疗中补充ALA可能对患有胃肠道黏膜炎的癌症患者起到保护干预作用。根据研究结果，ALA作为一种具有多种生物学特性的食物源性抗氧化剂，可能是治疗5-FU诱导的胃肠道黏膜炎的有效方法，并可预防接受5-FU治疗的癌症患者的氧化应激、炎症和组织损伤。
