Cai Jiaming, Xing Lujuan, Zhang Wangang, Fu Lijuan, Zhang Jian
Key Laboratory of Meat Processing and Quality Control, MOE, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Antioxidants (Basel). 2022 Sep 30;11(10):1970. doi: 10.3390/antiox11101970.
The aim of this study was to select potential probiotic yeasts from dry-cured Xuanwei ham and investigate yeast-derived antioxidant peptides. The results showed that two strains (XHY69 and XHY79) were selected as potential probiotic yeasts and identified as Yamadazyma triangularis. The two yeasts showed tolerance under pH 2.5 and 1% bile salt, in addition to protease activity, auto-aggregation, antibacterial, and antioxidant activities. The peptide fraction (MW < 3 kDa) isolated from XHY69 fermentation broth, named XHY69AP, showed higher radical scavenging activities than glutathione at a concentration of 4.5 mg/mL (p < 0.05). The fraction (AP-D10) was purified from XHY69AP by gel filtration chromatography and reversed-phase high performance liquid chromatography, and then further identified by a UHPLC-LTQ-Orbitrap mass spectrometer. The molecular weight of all 55 purified sequences was distributed between 0.370 and 0.735 kDa. Among these seven novel peptides, Tyr-Pro-Leu-Pro (YPLP), Ala-Gly-Pro-Leu (AGPL), Gly-Pro-Phe-Pro (GPFP), and Ala-Pro-Gly-Gly-Phe (APGGF) were identified. All sequences were abundant in hydrophobic amino acids, especially proline residue. Among these novel peptides, YPLP possessed the highest ABTS scavenging rate (75.48%). The present work selects two new probiotic potential yeasts from dry-cured Xuanwei ham that are effective to yield novel antioxidant peptides.
本研究的目的是从宣威火腿中筛选潜在的益生菌酵母，并研究酵母来源的抗氧化肽。结果表明，筛选出两株潜在的益生菌酵母（XHY69和XHY79），并鉴定为三角山梨酵母。这两株酵母在pH 2.5和1%胆盐条件下具有耐受性，同时还具有蛋白酶活性、自聚集、抗菌和抗氧化活性。从XHY69发酵液中分离得到的肽段（分子量<3 kDa），命名为XHY69AP，在浓度为4.5 mg/mL时，其自由基清除活性高于谷胱甘肽（p<0.05）。通过凝胶过滤色谱和反相高效液相色谱从XHY69AP中纯化得到组分（AP-D10），然后用超高效液相色谱-线性离子阱-轨道阱质谱仪进一步鉴定。所有55条纯化序列的分子量分布在0.370至0.735 kDa之间。在这七种新型肽中，鉴定出了酪氨酰-脯氨酰-亮氨酰-脯氨酸（YPLP）、丙氨酰-甘氨酰-脯氨酰-亮氨酸（AGPL）、甘氨酰-脯氨酰-苯丙氨酰-脯氨酸（GPFP）和丙氨酰-脯氨酰-甘氨酰-甘氨酰-苯丙氨酸（APGGF）。所有序列中疏水氨基酸含量丰富，尤其是脯氨酸残基。在这些新型肽中，YPLP的ABTS清除率最高（75.48%）。本研究从宣威火腿中筛选出两株具有产生新型抗氧化肽潜力的新的益生菌酵母。
