Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Biosensors (Basel). 2022 Sep 25;12(10):788. doi: 10.3390/bios12100788.
Cholesterol, an important lipid in animal membranes, binds to hydrophobic pockets within many soluble proteins, transport proteins and membrane bound proteins. The study of cholesterol-protein interactions in aqueous solutions is complicated by cholesterol's low solubility and often requires organic co-solvents or surfactant additives. We report the synthesis of a biotinylated cholesterol and immobilization of this derivative on a streptavidin chip. Surface plasmon resonance (SPR) was then used to measure the kinetics of cholesterol interaction with cholesterol-binding proteins, hedgehog protein and tyrosine phosphatase 1B.
胆固醇是动物膜中的一种重要脂质，它与许多可溶性蛋白、转运蛋白和膜结合蛋白中的疏水口袋结合。在水溶液中研究胆固醇-蛋白质相互作用比较复杂，因为胆固醇的溶解度低，通常需要有机溶剂或表面活性剂添加剂。我们报告了一种生物素化胆固醇的合成，并将该衍生物固定在链霉亲和素芯片上。然后，表面等离子体共振（SPR）用于测量胆固醇与胆固醇结合蛋白、 hedgehog 蛋白和酪氨酸磷酸酶 1B 相互作用的动力学。
