State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
Biosensors (Basel). 2022 Oct 6;12(10):832. doi: 10.3390/bios12100832.
In this work, a label-free fluorescent detection method for glyphosate, based on DNA-templated silver nanoclusters (DNA-Ag NCs) and a Cu-ion-modulated strategy, was developed. In the presence of Cu, the fluorescence of the DNA-Ag NCs was quenched. Glyphosate can restore the fluorescence of DNA-Ag NCs. By analyzing the storage stability of the obtained DNA-Ag NCs using different DNA templates, specific DNA-Ag NCs were selected for the construction of the glyphosate sensor. The ultrasensitive detection of glyphosate was achieved by optimizing the buffer pH and Cu concentration. The sensing of glyphosate demonstrated a linear response in the range of 1.0-50 ng/mL. The limit of detection (LOD) was 0.2 ng/mL. The proposed method was successfully applied in the detection of glyphosate in a real sample, indicating its high application potential for glyphosate detection.
本工作开发了一种基于 DNA 模板银纳米簇（DNA-Ag NCs）和 Cu 离子调控策略的草甘膦无标记荧光检测方法。在 Cu 存在的情况下，DNA-Ag NCs 的荧光被猝灭。草甘膦可以恢复 DNA-Ag NCs 的荧光。通过分析不同 DNA 模板对所得 DNA-Ag NCs 存储稳定性的影响，选择了特定的 DNA-Ag NCs 用于构建草甘膦传感器。通过优化缓冲液 pH 值和 Cu 浓度，实现了对草甘膦的超灵敏检测。草甘膦的传感在 1.0-50ng/mL 的范围内呈现线性响应。检测限（LOD）为 0.2ng/mL。该方法成功应用于实际样品中草甘膦的检测，表明其在草甘膦检测方面具有很高的应用潜力。
