Functional Genomics Laboratory, Department of Biosciences, COMSATS University Islamabad, Islamabad 45550, Pakistan.
Department of Biochemistry, Usmanu Danfodiyo University, Sokoto P.M.B. 2346, Nigeria.
Genes (Basel). 2022 Sep 22;13(10):1704. doi: 10.3390/genes13101704.
Lipotoxicity is known to cause cellular dysfunction and death in non-adipose tissue. A major cause of lipotoxicity is the accumulation of saturated free fatty acids (FFA). Palmitic acid (PA) is the most common saturated fatty acid found in the human body. Endothelial cells form the blood vessels and are the first non-adipose cells to encounter FFA in the bloodstream. FFA overload has a direct impact on metabolism, which is evident through the changes occurring in mitochondria. To study these changes, the PA-treated human coronary artery endothelial cell (HCAEC) dataset was obtained from the Gene Expression Omnibus (GEO), and it was analyzed to obtain differentially expressed genes (DEGs) from the nucleus and mitochondria. Functional and pathway enrichment analyses were performed on DEGs. Results showed that nuclear and mitochondrial DEGs were implicated in several processes, e.g., reactive oxygen species (ROS) production, mitochondrial fusion and fission, Ca sequestering, membrane transport, the electron transport chain and the process of apoptosis. To better understand the role of FFA in endothelial cell damage, these DEGs can lead to future experiments based on these findings.
脂毒性已知会导致非脂肪组织中的细胞功能障碍和死亡。脂毒性的一个主要原因是饱和游离脂肪酸 (FFA) 的积累。棕榈酸 (PA) 是人体内最常见的饱和脂肪酸。内皮细胞形成血管，是血液中首先遇到 FFA 的非脂肪细胞。FFA 过载对代谢有直接影响，这可以从线粒体发生的变化中明显看出。为了研究这些变化，从基因表达综合数据库 (GEO) 中获取了经 PA 处理的人冠状动脉内皮细胞 (HCAEC) 数据集，并对其进行了分析，以从细胞核和线粒体中获得差异表达基因 (DEG)。对 DEG 进行了功能和途径富集分析。结果表明，细胞核和线粒体 DEG 与多种过程有关，例如活性氧 (ROS) 产生、线粒体融合和裂变、Ca 离子储存、膜转运、电子传递链和细胞凋亡过程。为了更好地了解 FFA 在血管内皮细胞损伤中的作用，这些 DEG 可以为基于这些发现的未来实验提供依据。
