College of Life and Health Sciences, Northeastern University, Shenyang 110004, China.
Genes (Basel). 2022 Sep 25;13(10):1720. doi: 10.3390/genes13101720.
, which belongs to the Anthemideae (Asteraceae), is widely cultivated as an ornamental plant. In this study, the complete chloroplast genome of was obtained based on the sequences generated by Illumina HiSeq. The chloroplast genome of was 149,626 base pairs (bp) in length, containing a pair of inverted repeats (IR, 24,510 bp) regions separated by a small single-copy (SSC, 18,352 bp) sequence and a large single-copy (LSC, 82,254 bp) sequence. The genome contained 132 genes, consisting of 85 coding DNA sequences, 37 tRNA genes, and 8 rRNA genes, with nineteen genes duplicated in the IR region. A comparison chloroplast genome analysis among ten species from the tribe of Anthemideae revealed that the chloroplast genome size varied, but the genome structure, gene content, and oligonucleotide repeats were highly conserved. Highly divergent regions, e.g., , -, - intronic, were detected. Phylogenetic analysis supported as a separate genus. The findings of this study will be helpful in the exploration of the phylogenetic relationships of the tribe of Anthemideae and contribute to the breeding improvement of .
万寿菊,属于菊科(Asteraceae)的春黄菊属(Anthemideae),作为观赏植物被广泛种植。本研究基于 Illumina HiSeq 产生的序列,获得了万寿菊的完整叶绿体基因组。万寿菊的叶绿体基因组大小为 149626bp,包含一对反向重复(IR)区(24510bp),由一个小单拷贝(SSC)区(18352bp)和一个大单拷贝(LSC)区(82254bp)序列隔开。基因组包含 132 个基因,由 85 个编码 DNA 序列、37 个 tRNA 基因和 8 个 rRNA 基因组成,其中 19 个基因在 IR 区重复。对春黄菊族 10 个物种的叶绿体基因组比较分析表明,叶绿体基因组大小存在差异,但基因组结构、基因含量和寡核苷酸重复高度保守。检测到高度变异区域,如,, -内含子。系统发育分析支持将万寿菊作为一个独立的属。本研究结果将有助于探索春黄菊族的系统发育关系,并为万寿菊的育改良提供参考。