Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing 210037, China.
Department of Forest Resources Management, Faculty of Science, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Genes (Basel). 2022 Oct 6;13(10):1803. doi: 10.3390/genes13101803.
Abiotic stresses such as salt stress seriously affect the growth and yield of plants. Lcdcb () is a widely cultivated halophyte in saline-alkali areas of the world. As an essential element for plant growth and development, K plays an irreplaceable role in improving the tolerance of plants to salt stress. However, there are few reports on the mechanism of K in promoting plant hormones to reduce the damage of NaCl stress to . In this study, we sequenced the transcriptome of the roots of which were treated with exogenous potassium (K) for 0 h, 48 h and 168 h under NaCl stress, according to the changes in the expression levels of differentially expressed genes (DEGs) in roots. Key candidate genes and metabolic pathways related to plant hormones were mined for analysis and further verified by quantitative real-time PCR (qRT-PCR). The results showed that under NaCl stress for 48 h and 168 h, there were a large number of DEGs in the roots of , and the expression levels changed over time. In particular, we found that 56 plant hormone-related genes were annotated to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and with the increase of time, their expression levels were mainly up-regulated and involved in the related metabolic pathways to resist NaCl stress. It is worth noting that 7 DEGs related to abscisic acid (ABA), 28 DEGs related to auxin, 1 DEG related to ethylene (ET), and 1 DEG related to cytokinin (CK) were added within 168 h of exogenous potassium, and they were involved in alleviating the root damage of under NaCl stress and played an important role. In addition, we found the plant hormone signal transduction pathway, which plays an important role in resistance to NaCl stress. As a result of this study, the molecular mechanism of plant hormones involved in applying exogenous potassium under NaCl stress is further understood, resulting in a better understanding of how exogenous potassium can alleviate the damage caused by NaCl under stress in .
非生物胁迫，如盐胁迫，严重影响植物的生长和产量。盐角草是世界上盐碱地广泛种植的盐生植物。钾作为植物生长发育所必需的元素，在提高植物耐盐性方面发挥着不可替代的作用。然而，关于 K 促进植物激素来减轻 NaCl 胁迫对 的损伤的机制的报道很少。在这项研究中，我们根据盐胁迫下外源钾（K）处理 0 h、48 h 和 168 h 后根中差异表达基因（DEGs）的表达水平，对其进行了转录组测序。对与植物激素相关的关键候选基因和代谢途径进行了挖掘和分析，并通过定量实时 PCR（qRT-PCR）进一步验证。结果表明，在 NaCl 胁迫下 48 h 和 168 h， 根中存在大量 DEGs，且表达水平随时间变化。特别是，我们发现 56 个与植物激素相关的基因被注释到京都基因与基因组百科全书（KEGG）通路，随着时间的增加，它们的表达水平主要上调，并参与了相关的代谢途径以抵抗 NaCl 胁迫。值得注意的是，在 168 h 内添加了 7 个与脱落酸（ABA）相关的 DEGs、28 个与生长素相关的 DEGs、1 个与乙烯（ET）相关的 DEG 和 1 个与细胞分裂素（CK）相关的 DEG，它们参与缓解 NaCl 胁迫下 根的损伤，并发挥重要作用。此外，我们发现了植物激素信号转导途径，该途径在耐 NaCl 胁迫中起着重要作用。本研究进一步了解了植物激素在施加外源钾缓解 NaCl 胁迫下损伤的分子机制，更好地理解了外源钾如何在胁迫下减轻盐对 的损伤。
