Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing, Jiangsu, China.
Department of Forest Resources Management, University of British Columbia, Vancouver, British Columbia, Canada.
PLoS One. 2022 Mar 31;17(3):e0265653. doi: 10.1371/journal.pone.0265653. eCollection 2022.
Halophyte Tamarix ramosissima. Lcdcb (T. ramosissima) are known as the representative of Tamarix plants that are widely planted in salinized soil. However, molecular mechanisms towards salt tolerance and adaptation are largely rare. In this study, we carried out RNA-sequence and transcriptome analysis of T. ramosissima in response to NaCl stress, screened differentially expressed genes (DEGs) and further verified by qRT-PCR. Results showed that 105702 unigenes were spliced from the raw data of transcriptome sequencing, where 54238 unigenes were retrieved from KEGG, KOG, NR, and SwissProt. After 48 hours of NaCl treatment, the expression levels of 6374 genes were increased, and 5380 genes were decreased in leaves. After 168 hours, the expression levels of 3837 genes were up-regulated and 7808 genes were down-regulated. In particular, 8 transcription factors annotated to the KEGG Pathway were obtained, involving the WRKY and bZIP transcription family. In addition, KEGG pathway annotation showed that expression of 39 genes involved in ROS scavenging mechanisms were significantly changed, in which 21 genes were up-regulated and 18 genes were down-regulated after 48 hours as well as 15 genes were up-regulated and 24 genes were down-regulated after 168h. Simultaneously, the enzyme activities of SOD and POD were significantly enhanced under NaCl treatment, but the enzyme activity of CAT was not significantly enhanced. Moreover, WRKY, MYB and bZIP may participate in the process of salt resistance in T. ramosissima. This study provides gene resources and a theoretical basis for further molecular mechanisms of salt tolerance in T. ramosissima.
盐生植物柽柳（Tamarix ramosissima）。Lcdcb（柽柳）是广泛种植在盐渍土壤中的柽柳植物的代表。然而，关于耐盐和适应的分子机制在很大程度上还不清楚。在这项研究中，我们对柽柳在盐胁迫下进行了 RNA-seq 和转录组分析，筛选了差异表达基因（DEGs），并通过 qRT-PCR 进一步验证。结果表明，从转录组测序的原始数据中拼接出 105702 个基因，其中 54238 个基因从 KEGG、KOG、NR 和 SwissProt 中检索到。经 48 小时 NaCl 处理后，叶片中 6374 个基因的表达水平升高，5380 个基因的表达水平降低。168 小时后，3837 个基因的表达水平上调，7808 个基因的表达水平下调。特别是获得了 8 个注释到 KEGG 途径的转录因子，涉及 WRKY 和 bZIP 转录家族。此外，KEGG 途径注释显示，参与 ROS 清除机制的 39 个基因的表达发生了显著变化，其中 48 小时后 21 个基因上调，18 个基因下调，168 小时后 15 个基因上调，24 个基因下调。同时，NaCl 处理下 SOD 和 POD 的酶活性显著增强，但 CAT 的酶活性没有显著增强。此外，WRKY、MYB 和 bZIP 可能参与了柽柳的耐盐过程。本研究为进一步研究柽柳耐盐的分子机制提供了基因资源和理论依据。
