Key Laboratory of Environmental Pollution and Integrative Omics, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Huan Cheng North 2nd Road 109, Guilin 541004, China.
Department of Chemistry, City University of Hong Kong, Hong Kong SAR, China.
Int J Mol Sci. 2022 Oct 17;23(20):12417. doi: 10.3390/ijms232012417.
Fish gills are the major osmoregulatory tissue that contact the external water environment and have developed an effective osmoregulatory mechanism to maintain cellular function. Marine medaka () has the ability to live in both seawater and fresh water environments. The present study performed a seawater (SW) to 50% seawater (SFW) transfer, and the gill samples were used for comparative transcriptomic analysis to study the alteration of hypo-osmotic stress on immune responsive genes in this model organism. The result identified 518 differentiated expressed genes (DEGs) after the SW to SFW transfer. Various pathways such as p53 signaling, forkhead box O signaling, and the cell cycle were enriched. Moreover, the immune system was highlighted as one of the top altered biological processes in the enrichment analysis. Various cytokines, chemokines, and inflammatory genes that participate in the IL-17 signaling pathway were suppressed after the SW to SFW transfer. On the other hand, some immunoglobulin-related genes were up-regulated. The results were further validated by real-time qPCR. Taken together, our study provides additional gill transcriptome information in marine medaka; it also supports the notion that osmotic stress could influence the immune responses in fish gills.
鱼类的鳃是主要的渗透调节组织，与外部水环境接触，并发展出有效的渗透调节机制来维持细胞功能。海水型斑马鱼（）有生活在海水和淡水中的能力。本研究进行了海水（SW）到 50%海水（SFW）的转移，并用鳃样本进行比较转录组分析，以研究这种模式生物在低渗胁迫下免疫应答基因的变化。结果在 SW 到 SFW 转移后鉴定出 518 个差异表达基因（DEGs）。富集到了各种途径，如 p53 信号、叉头框 O 信号和细胞周期。此外，免疫系统在富集分析中被突出为改变最大的生物学过程之一。参与白细胞介素-17 信号通路的各种细胞因子、趋化因子和炎症基因在 SW 到 SFW 转移后被抑制。另一方面，一些免疫球蛋白相关基因被上调。实时 qPCR 进一步验证了这些结果。总之，我们的研究为海水型斑马鱼的鳃提供了额外的转录组信息；它还支持渗透压应激会影响鱼类鳃中免疫反应的观点。
