Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Jiangsu 214081, China.
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Jiangsu 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu 214081, China.
Ecotoxicol Environ Saf. 2021 Dec 15;226:112848. doi: 10.1016/j.ecoenv.2021.112848. Epub 2021 Oct 4.
Salinity changes will threaten the survival of aquatic animals. However, osmoregulatory mechanism of Coilia nasus has not been explored. Oxford Nanopore Technologies (ONT) sequencing was performed in C. nasus gills during hypotonic and hyperosmotic stress. 23.8 G clean reads and 27,659 full-length non-redundant sequences were generated via ONT sequencing. Alternative splicing, alternative polyadenylation, transcript factors, and long noncoding RNA were identified. During hypotonic stress, 58 up-regulated differentially expressed genes (DEGs) and 36 down-regulated DEGs were identified. During hypertonic stress, 429 up-regulated DEGs and 480 down-regulated DEGs were identified. These DEGs were associated with metabolism, cell cycle, and transport. The analysis of these DEGs indicated that carbohydrate and fatty acid metabolism were activated to provide energy for cell cycle and transport during hypotonic and hypertonic stress. Cell cycle was also promoted during hypotonic and hypertonic stress. To resist hypotonic stress, polyamines metabolism, ion absorption and water transport from extra-cellular to intra-cellular were promoted, while ion secretion was inhibited. During hypotonic stress, glutamine, alanine, proline, and inositol metabolism were activated. Ion absorption and water transport from intra-cellular to extra-cellular were inhibited. Moreover, different transcript isoforms generated from the same gene performed different expression patterns during hypotonic and hypertonic stress. These findings will be beneficial to understand osmoregulatory mechanism of Coilia nasus.
盐度变化将威胁水生动物的生存。然而，关于 Coilia nasus 的渗透调节机制尚未被探索。在 Coilia nasus 的鳃中进行了低渗和高渗胁迫的 Oxford Nanopore Technologies（ONT）测序。ONT 测序产生了 23.8 G 的清洁读数和 27659 条全长非冗余序列。鉴定了可变剪接、可变多聚腺苷酸化、转录因子和长非编码 RNA。在低渗胁迫下，鉴定出 58 个上调差异表达基因（DEG）和 36 个下调 DEG。在高渗胁迫下，鉴定出 429 个上调 DEG 和 480 个下调 DEG。这些 DEG 与代谢、细胞周期和运输有关。这些 DEG 的分析表明，在低渗和高渗胁迫下，碳水化合物和脂肪酸代谢被激活，为细胞周期和运输提供能量。细胞周期也在低渗和高渗胁迫下得到促进。为了抵抗低渗胁迫，促进了多胺代谢、离子吸收和水从细胞外到细胞内的运输，同时抑制了离子分泌。在低渗胁迫下，谷氨酰胺、丙氨酸、脯氨酸和肌醇代谢被激活。离子吸收和水从细胞内到细胞外的运输受到抑制。此外，同一基因的不同转录异构体在低渗和高渗胁迫下表现出不同的表达模式。这些发现将有助于理解 Coilia nasus 的渗透调节机制。
