Department of Cardiovascular Biomedicine, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, Tlalpan, Ciudad de México 14080, Mexico.
Department de and Hysiology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, Tlalpan, Ciudad de México 14080, Mexico.
Int J Mol Sci. 2022 Oct 19;23(20):12529. doi: 10.3390/ijms232012529.
Deodorized garlic (DG) may favor the activity of the antioxidant enzymes and promote the synthesis of hydrogen sulfide (HS). The objective was to test if DG favors an increase in HS and if it decreases the oxidative stress caused by lipopolysaccharide (LPS) in rat hearts. A total of 24 rats were divided into 4 groups: Group 1 control (C), Group 2 LPS, Group 3 DG, and Group 4 LPS plus DG. The cardiac mechanical performance (CMP), coronary vascular resistance (CVR), and oxidative stress markers, such as total antioxidant capacity (TAC), glutathione (GSH), selenium (Se), lipid peroxidation (LPO), thiols, hydrogen sulfide (HS), and the activities and expressions of thioredoxin reductase (TrxR), glutathione peroxidase (GPx), and glutathione-S-transferase (GST), cystathionine synthetase (CBS), cystathionine γ-lyase (CTH), iNOS, and eNOS-p, were analyzed in the heart. Infarct zones in the cardiac tissue were present ( = 0.01). The CMP and CVR decreased and increased ( ≤ 0.05), TAC, GSH, HS, NO, thiols, and GST activity ( ≤ 0.01) decreased, and LPO and iNOS increased ( ≤ 0.05). The activities and expressions of TrxR, GPx, eNOS-, CTH, and CBS ( ≤ 0.05) decreased with the LPS treatment; however, DG normalized this effect. DG treatment decreases heart damage caused by LPS through the cross-talk between the HS and NO systems.
脱臭大蒜（DG）可能有利于抗氧化酶的活性，并促进硫化氢（HS）的合成。目的是测试 DG 是否有利于增加 HS，并降低 LPS 对大鼠心脏造成的氧化应激。总共 24 只大鼠分为 4 组：第 1 组对照组（C）、第 2 组 LPS 组、第 3 组 DG 组和第 4 组 LPS+DG 组。分析心脏的心肌力学性能（CMP）、冠状血管阻力（CVR）和氧化应激标志物，如总抗氧化能力（TAC）、谷胱甘肽（GSH）、硒（Se）、脂质过氧化（LPO）、硫醇、硫化氢（HS）以及硫氧还蛋白还原酶（TrxR）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽-S-转移酶（GST）的活性和表达、胱硫醚合成酶（CBS）、胱硫醚γ-裂合酶（CTH）、诱导型一氧化氮合酶（iNOS）和内皮型一氧化氮合酶（eNOS-p）。心脏组织中存在梗塞区（=0.01）。CMP 和 CVR 降低，增加（≤0.05），TAC、GSH、HS、NO、硫醇和 GST 活性降低（≤0.01），LPO 和 iNOS 增加（≤0.05）。LPS 处理后，TrxR、GPx、eNOS-、CTH 和 CBS 的活性和表达降低（≤0.05）；然而，DG 使这种作用正常化。DG 治疗通过 HS 和 NO 系统的相互作用降低 LPS 引起的心脏损伤。
