Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34 000 Kragujevac, Serbia.
Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34 000 Kragujevac, Serbia.
Int J Mol Sci. 2020 Nov 30;21(23):9100. doi: 10.3390/ijms21239100.
This study aimed to examine the effects of diallyl trisulfide (DATS), the most potent polysulfide derived from garlic, on metabolic syndrome and myocardial function in rats with metabolic syndrome (MetS). For that purpose, we used 36 male rats divided into control rats, rats with MetS and MetS rats treated with 40 mg/kg of DATS every second day for 3 weeks. In the first part, we studied the impact of DATS on MetS control and found that DATS significantly raised HS, decreased homocysteine and glucose levels and enhanced lipid and antioxidative, while reducing prooxidative parameters. Additionally, this polysulfide improved cardiac function. In the second part, we investigated the impact of DATS on ex vivo induced ischemia/reperfusion (I/R) heart injury and found that DATS consumption significantly improved cardiodynamic parameters and prevented oxidative and histo-architectural variation in the heart. In addition, DATS significantly increased relative gene expression of eNOS, SOD-1 and -2, Bcl-2 and decreased relative gene expression of NF-κB, IL-17A, Bax, and caspases-3 and -9. Taken together, the data show that DATS can effectively mitigate MetS and have protective effects against ex vivo induced myocardial I/R injury in MetS rat.
本研究旨在探讨二烯丙基三硫醚（DATS）——大蒜中最强的多硫化物对代谢综合征大鼠代谢综合征和心肌功能的影响。为此，我们使用 36 只雄性大鼠，分为对照组大鼠、代谢综合征大鼠和每天用 40mg/kg DATS 处理的代谢综合征大鼠，共处理 3 周。在第一部分中，我们研究了 DATS 对代谢综合征的影响，发现 DATS 可显著提高 HS、降低同型半胱氨酸和葡萄糖水平，并增强脂质和抗氧化能力，同时降低促氧化参数。此外，这种多硫化物还改善了心脏功能。在第二部分中，我们研究了 DATS 对离体诱导的缺血/再灌注（I/R）心脏损伤的影响，发现 DATS 的摄入可显著改善心脏动力学参数，并防止心脏的氧化和组织学变化。此外，DATS 还显著增加了 eNOS、SOD-1 和 SOD-2、Bcl-2 的相对基因表达，降低了 NF-κB、IL-17A、Bax 和 caspase-3 和 caspase-9 的相对基因表达。总之，这些数据表明 DATS 可有效减轻代谢综合征，并对代谢综合征大鼠离体诱导的心肌 I/R 损伤具有保护作用。
