College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Key Laboratory of Protected Horticulture Ministry of Education, Shenyang 110866, China.
Int J Mol Sci. 2022 Oct 19;23(20):12537. doi: 10.3390/ijms232012537.
Root-zone CO is a major factor that affects crop growth, development, nutrient uptake, and metabolism. Oriental melon is affected by root-zone gases during growth, the microstructure, sugar and starch contents, enzymatic activities related to sugar and starch metabolism, and gene expression in the roots of oriental melon seedlings were investigated under three root-zone CO concentrations (CK: 0.2%, T1: 0.4%, T2: 1.1%). Elevated root-zone CO altered the cellular microstructure, accelerated the accumulation and release of starch grains, disrupted organelle formation, and accelerated root senescence. The sugar and starch contents and metabolic activity in the roots increased within a short duration following treatment. Compared to the control, 232 and 1492 differentially expressed genes (DEGs) were identified on the 6th day of treatment in T1 and T2 plants, respectively. The DEGs were enriched in three metabolic pathways. The majority of genes related to sucrose and starch hydrolysis were upregulated, while the genes related to sucrose metabolism were downregulated. The study revealed that oriental melon seedlings adapt to elevated root-zone CO stress by adjusting sugar and starch metabolism at the transcriptome level and provides new insights into the molecular mechanism underlying the response to elevated root-zone CO stress.
根区 CO 是影响作物生长、发育、养分吸收和代谢的主要因素。在三种根区 CO 浓度（CK：0.2%、T1：0.4%、T2：1.1%）下，研究了东方甜瓜在生长过程中受根区气体影响时，幼苗根系的微观结构、糖和淀粉含量、与糖和淀粉代谢相关的酶活性以及基因表达。根区 CO 升高改变了细胞的微观结构，加速了淀粉颗粒的积累和释放，破坏了细胞器的形成，加速了根系衰老。处理后短时间内，根中的糖和淀粉含量以及代谢活性增加。与对照相比，T1 和 T2 处理第 6 天分别鉴定出 232 和 1492 个差异表达基因（DEGs）。DEGs 富集在三个代谢途径中。与蔗糖和淀粉水解相关的大多数基因上调，而与蔗糖代谢相关的基因下调。该研究揭示了东方甜瓜幼苗通过在转录组水平上调节糖和淀粉代谢来适应升高的根区 CO 胁迫，并为响应升高的根区 CO 胁迫的分子机制提供了新的见解。
