Lake Janice A, Walker Heather J, Cameron Duncan D, Lomax Barry H
Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK.
School of Biosciences, University of Nottingham, Loughborough LE12 5RD, UK.
Physiol Plant. 2017 Apr;159(4):433-444. doi: 10.1111/ppl.12525. Epub 2016 Nov 23.
Investigations were undertaken in the context of the potential environmental impact of carbon capture and storage (CCS) transportation in the form of a hypothetical leak of extreme levels of CO into the soil environment and subsequent effects on plant physiology. Laboratory studies using purpose built soil chambers, separating and isolating the soil and aerial environments, were used to introduce high levels of CO gas exclusively into the rhizosphere. CO concentrations greater than 32% in the isolated soil environment revealed a previously unknown whole plant stomatal response. Time course measurements of stomatal conductance (g ), leaf temperature and leaf abscisic acid (ABA) show strong coupling between all three variables over a specific period (3 h following CO gassing) occurring as a result of CO -specific detection by roots. The coupling of g and ABA subsequently breaks down resulting in a rapid and complete loss of turgor in the shoot. Root access to water is severely restricted as evidenced by the inability to counter turgor loss, however, the plant regains some turgor over time. Recovery of full turgor is not achieved over the longer term. Results suggest an immediate perception and whole plant response as changes in measured parameters (leaf temperature, g and ABA) occur in the shoot, but the response is solely due to detection of very high CO concentration at the root/soil interface which results in loss of stomatal regulation and disruption to control over water uptake.
以假设极高水平的一氧化碳泄漏到土壤环境中以及随后对植物生理产生影响的形式，对碳捕获与封存（CCS）运输的潜在环境影响进行了调查。利用专门建造的土壤箱进行实验室研究，将土壤和地上环境分离并隔离，以便将高浓度的一氧化碳气体专门引入根际。在隔离的土壤环境中，一氧化碳浓度大于32%时，揭示了一种此前未知的整株植物气孔反应。气孔导度（g）、叶片温度和叶片脱落酸（ABA）的时间进程测量表明，在特定时间段（一氧化碳通气后3小时）内，由于根部对一氧化碳的特异性检测，这三个变量之间存在强烈的耦合关系。随后，g与ABA之间的耦合关系被打破，导致地上部分迅速完全丧失膨压。无法对抗膨压丧失表明根部获取水分受到严重限制，然而，随着时间的推移，植物会恢复一些膨压。从长期来看，无法完全恢复到满膨压状态。结果表明，当地上部分测量参数（叶片温度、g和ABA）发生变化时，会立即产生感知并引发整株植物反应，但这种反应完全是由于在根/土壤界面检测到非常高的一氧化碳浓度，导致气孔调节丧失以及对水分吸收控制的破坏。
