Kabtani Jihane, Militello Muriel, Ranque Stéphane
Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France.
Faculty of Medical and Paramedical Sciences, Aix-Marseille Université, AP-HM, IRD, SSA, MEPHI, 13005 Marseille, France.
J Fungi (Basel). 2022 Sep 23;8(10):999. doi: 10.3390/jof8100999.
The genus belongs to the class Ascomycota and the family Coniochaetaceae. Some of the species are plant and animal pathogens, while others are known to be primarily involved in human diseases. In the last few decades, case reports of human infections with have increased, mainly in immunocompromised hosts. We have described and characterised a new species in the genus , here named (PMML0158), which was isolated from a clinical sample. Species identification and thorough description were based on apposite and reliable phylogenetic and phenotypic approaches. The phylogenetic methods included multilocus phylogenetic analyses of four genomic regions: ITS (rRNA Internal Transcribed Spacers 1 and 2), TEF-1α (Translation Elongation Factor-1alpha), B-tub2 (β-tubulin2), and D1/D2 domains (28S large subunit rRNA). The phenotypic characterisation consisted, first, of a physiological analysis using both EDX (energy-dispersive X-ray spectroscopy) and Biolog advanced phenotypic technology for fixing the chemical mapping and carbon-source oxidation/assimilation profiles. Afterwards, morphological characteristics were highlighted by optical microscopy and scanning electron microscopy. The in vitro antifungal susceptibility profile was characterised using the E-test exponential gradient method. The molecular analysis revealed the genetic distance between the novel species (PMML0158) and other known taxa, and the phenotypic analysis confirmed its unique chemical and physiological profile when compared with all other species of this genus.
该属属于子囊菌门和座壳孢科。该属的一些物种是植物和动物病原体，而其他一些物种则主要与人类疾病有关。在过去几十年中，该属人类感染的病例报告有所增加，主要发生在免疫功能低下的宿主中。我们在此描述并鉴定了该属中的一个新物种，命名为（PMML0158），它是从一份临床样本中分离出来的。物种鉴定和详细描述基于恰当且可靠的系统发育和表型方法。系统发育方法包括对四个基因组区域进行多位点系统发育分析：ITS（核糖体RNA内部转录间隔区1和2）、TEF-1α（翻译延伸因子-1α）、B-tub2（β-微管蛋白2）和D1/D2结构域（28S大亚基核糖体RNA）。表型特征鉴定首先包括使用能量色散X射线光谱法（EDX）和Biolog先进表型技术进行生理分析，以确定化学图谱和碳源氧化/同化谱。之后，通过光学显微镜和扫描电子显微镜突出显示形态特征。使用E-test指数梯度法表征体外抗真菌药敏谱。分子分析揭示了新物种（PMML0158）与其他已知分类群之间的遗传距离，表型分析证实了与该属所有其他物种相比，它具有独特的化学和生理特征。
