sp. nov. Isolated from a Clinical Sampl28.

The genus belongs to the class Ascomycota and the family Coniochaetaceae. Some of the species are plant and animal pathogens, while others are known to be primarily involved in human diseases. In the last few decades, case reports of human infections with have increased, mainly in immunocompromised hosts. We have described and characterised a new species in the genus , here named (PMML0158), which was isolated from a clinical sample. Species identification and thorough description were based on apposite and reliable phylogenetic and phenotypic approaches. The phylogenetic methods included multilocus phylogenetic analyses of four genomic regions: ITS (rRNA Internal Transcribed Spacers 1 and 2), TEF-1α (Translation Elongation Factor-1alpha), B-tub2 (β-tubulin2), and D1/D2 domains (28S large subunit rRNA). The phenotypic characterisation consisted, first, of a physiological analysis using both EDX (energy-dispersive X-ray spectroscopy) and Biolog advanced phenotypic technology for fixing the chemical mapping and carbon-source oxidation/assimilation profiles. Afterwards, morphological characteristics were highlighted by optical microscopy and scanning electron microscopy. The in vitro antifungal susceptibility profile was characterised using the E-test exponential gradient method. The molecular analysis revealed the genetic distance between the novel species (PMML0158) and other known taxa, and the phenotypic analysis confirmed its unique chemical and physiological profile when compared with all other species of this genus.