IHU Méditerranée Infection, 13005, Marseille, France.
AP-HM, IRD, SSA, MEPHI, Aix-Marseille Université, 13005, Marseille, France.
Mycopathologia. 2023 Dec;188(6):957-971. doi: 10.1007/s11046-023-00792-4. Epub 2023 Sep 20.
The majority of Candida species are known as non-pathogenic yeasts and rarely involved in human diseases. However, recently case reports of human infections caused by non-albicans Candida species have increased, mostly in immunocompromised hosts. Our study aimed to describe and characterize as thoroughly as possible, a new species of the Metschnikowia clade, named here Candida massiliensis (PMML0037), isolated from a clinical sample of human sputum. We targeted four discriminant genetic regions: "Internal Transcribed Spacers" of rRNA, D1/D2 domains (28S large subunit rRNA) and part of the genes encoding Translation Elongation Factor 1-α and β-tubulin2. The genetic data were compared to morphological characters, from scanning electron microscopy (TM 4000 Plus, SU5000), physiological, including the results of oxidation and assimilation tests of different carbon sources by the Biolog system, and chemical mapping by Energy-Dispersive X-ray Spectroscopy. Lastly, the in vitro antifungal susceptibility profile was performed using the E-test™ exponential gradient method. The multilocus analysis supported the genetic position of Candida massiliensis (PMML0037) as a new species of the Metschnikowia clade, and the phenotypic analysis highlighted its unique morphological and chemical profile when compared to the other Candida/Metschnikowia species included in the study.
大多数念珠菌物种被认为是无致病性的酵母，很少引起人类疾病。然而，最近由非白念珠菌念珠菌引起的人类感染病例报告有所增加，主要发生在免疫功能低下的宿主中。我们的研究旨在尽可能详细地描述和表征一个新的 Metschnikowia 分支物种，命名为 Candida massiliensis（PMML0037），该物种从人类痰的临床样本中分离出来。我们针对了四个有区别的遗传区域：rRNA 的“内部转录间隔区”、D1/D2 结构域（28S 大亚基 rRNA）和编码翻译延伸因子 1-α 和β-微管蛋白 2 的部分基因。遗传数据与形态特征（TM 4000 Plus、SU5000 的扫描电子显微镜）、生理特征（Biolog 系统不同碳源的氧化和同化测试结果）和通过能量色散 X 射线光谱进行的化学图谱进行了比较。最后，使用 E 试验™指数梯度法进行了体外抗真菌药敏谱测定。多基因分析支持 Candida massiliensis（PMML0037）作为 Metschnikowia 分支的一个新物种的遗传地位，表型分析突出了其与研究中包含的其他 Candida/Metschnikowia 物种相比独特的形态和化学特征。
