Kang Chae-Won, Park Miey, Lee Hae-Jeung
Institute for Aging and Clinical Nutrition Research, Gachon University, Seongnam 13120, Gyeonggi-do, Korea.
Department of Food and Nutrition, College of BioNano Technology, Gachon University, Seongnam 13120, Gyeonggi-do, Korea.
Life (Basel). 2022 Oct 18;12(10):1630. doi: 10.3390/life12101630.
Mulberry ( L.) leaves have been used to lower blood glucose in patients with diabetes. We evaluated the effects of mulberry leaves extract (MLE) and 1-deoxynojirimycin (1-DNJ) in improving insulin resistance through the activation of the IRS-1/PI3K/Akt pathway in the skeletal muscle of / mice. Histological analysis revealed an amelioration of muscle deformation and increased muscle fiber size. MLE and 1-DNJ positively raised the protein expression of related glucose uptake and increased the translocation of glucose transporter type 4 (GLUT4) to the membrane. Furthermore, MLE and 1-DNJ activated the IRS-1/PI3K/Akt pathway in the skeletal muscle and, subsequently, modulated the protein levels of glycogen synthase kinase-3beta (GSK-3β) and glycogen synthase (GS), leading to elevated muscle glycogen content. These findings suggest that MLE and 1-DNJ supplementation improves insulin resistance by modulating the insulin signaling pathway in the skeletal muscle of / mice.
桑叶已被用于降低糖尿病患者的血糖。我们评估了桑叶提取物（MLE）和1-脱氧野尻霉素（1-DNJ）通过激活/小鼠骨骼肌中IRS-1/PI3K/Akt信号通路来改善胰岛素抵抗的作用。组织学分析显示肌肉变形得到改善，肌纤维大小增加。MLE和1-DNJ使相关葡萄糖摄取的蛋白表达呈阳性，并增加了4型葡萄糖转运蛋白（GLUT4）向细胞膜的转位。此外，MLE和1-DNJ激活了骨骼肌中的IRS-1/PI3K/Akt信号通路，随后调节了糖原合酶激酶-3β（GSK-3β）和糖原合酶（GS）的蛋白水平，导致肌肉糖原含量升高。这些发现表明，补充MLE和1-DNJ可通过调节/小鼠骨骼肌中的胰岛素信号通路来改善胰岛素抵抗。
