Xuzhou Infectious Diseases Hospital, Xuzhou, 221000, Jiangsu, China.
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, 221000, Jiangsu, China.
BMC Complement Med Ther. 2021 Jun 14;21(1):172. doi: 10.1186/s12906-021-03342-x.
Mulberry leaf as a traditional Chinese medicine is able to treat obesity, diabetes, and dyslipidemia. It is well known that diabetes leads to intestinal microbiota dysbiosis. It is also recently discovered that liver glycogen structure is impaired in diabetic animals. Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day). Composition of intestinal microbiota was analyzed via metagenomics by sequencing the V3-V4 region of 16S rDNAs. Liver glycogen structure was characterized through size exclusion chromatography (SEC). Both Student's t-test and Tukey's test were used for statistical analysis.
A group of type 2 diabetic rat models were successfully established. Intestinal microbiota analysis showed that ethanol extract of mulberry leaves could partially change intestinal microbiota back to normal conditions. In addition, liver glycogen was restored from fragile state to stable state through administration of ethanol extract of mulberry leaves.
This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats. These finding can be helpful in discovering the novel therapeutic targets with the help of further investigations.
桑叶作为一种传统中药，能够治疗肥胖、糖尿病和血脂异常。众所周知，糖尿病会导致肠道微生物群落失调。最近还发现，糖尿病动物的肝糖原结构受损。由于桑叶能够通过降低血糖水平来改善糖尿病状况，因此研究其对肠道微生物群落和肝糖原结构是否有积极影响将是很有趣的。
在这项研究中，我们首先通过高效液相色谱（HPLC）和液相色谱/质谱（LC/MS）确定了桑叶乙醇提取物的生物活性成分。将动物模型分为三组，正常 Sprague-Dawley（SD）大鼠、高脂肪饮食（HFD）和链脲佐菌素（STZ）诱导的 2 型糖尿病大鼠，以及给予桑叶乙醇提取物（200mg/kg/天）的 HFD/STZ 诱导的大鼠。通过对 16S rDNAs 的 V3-V4 区进行测序，采用宏基因组学分析肠道微生物群落的组成。通过尺寸排阻色谱（SEC）对肝糖原结构进行表征。统计分析采用学生 t 检验和 Tukey 检验。
成功建立了一组 2 型糖尿病大鼠模型。肠道微生物群落分析表明，桑叶乙醇提取物可以部分使肠道微生物群落恢复正常状态。此外，通过给予桑叶乙醇提取物，肝糖原从脆弱状态恢复到稳定状态。
本研究证实，桑叶乙醇提取物（MLE）可改善糖尿病大鼠的肠道微生物群落失调，并增强肝糖原的脆弱性。这些发现有助于通过进一步研究发现新的治疗靶点。
Zotero 插件
