Guo Gang, Wu Xuanye, Liu Demeng, Liao Lingni, Zhang Di, Zhang Yi, Mao Tianjiao, He Yuhan, Huang Peng, Wang Wei, Su Lin, Wang Shuhua, Liu Qi, Ma Xingfeng, Shi Nan, Guan Yimin
Department of Microelectronics, Shanghai University, Shanghai 200000, China.
Shanghai Industrial μTechnology Research Institute, Shanghai 200000, China.
Micromachines (Basel). 2022 Sep 28;13(10):1620. doi: 10.3390/mi13101620.
Currently, many microchips must rely on an external force (such as syringe pump, electro-hydrodynamic pump, and peristaltic pump, etc.) to control the solution in the microchannels, which probably adds manual operating errors, affects the accuracy of fluid manipulation, and enlarges the noise of signal. In addition, the reasonable integration of micropump and microchip remain the stumbling block for the commercialization of microfluidic technique. To solve those two problems, we designed and fabricated a thermal bubble micropump based on MEMS (micro-electro-mechanical systems) technique. Many parameters (voltage, pulse time, cycle delay time, etc.) affecting the performance of this micropump were explored in this work. The experimental results showed the flow rate of solution with the assistance of a micropump reached more than 15 μL/min in the optimal condition. Finally, a method about measuring total aflatoxin in Chinese herbs was successfully developed based on the integrated platform contained competitive immunoassay and our micropump-based microfluidics. Additionally, the limit of detection in quantifying total aflatoxin (AF) was 0.0615 pg/mL in this platform. The data indicate this combined technique of biochemical assays and micropump based microchip have huge potential in automatically, rapidly, and sensitively measuring other low concentration of biochemical samples with small volume.
目前，许多微芯片必须依靠外力（如注射泵、电液动力泵和蠕动泵等）来控制微通道中的溶液，这可能会增加人工操作误差，影响流体操控的准确性，并增大信号噪声。此外，微泵与微芯片的合理集成仍然是微流控技术商业化的绊脚石。为了解决这两个问题，我们基于微机电系统（MEMS）技术设计并制造了一种热气泡微泵。在这项工作中，研究了许多影响该微泵性能的参数（电压、脉冲时间、循环延迟时间等）。实验结果表明，在最佳条件下，借助微泵的溶液流速达到了15 μL/min以上。最后，基于包含竞争免疫分析和我们基于微泵的微流控技术的集成平台，成功开发了一种测定中草药中总黄曲霉毒素的方法。此外，该平台定量总黄曲霉毒素（AF）的检测限为0.0615 pg/mL。数据表明，这种生化分析与基于微泵的微芯片相结合的技术在自动、快速、灵敏地测量其他低浓度小体积生化样品方面具有巨大潜力。
