Li Chen, Ye Bo, Xi Yongxin, Yuan Mu
College of Quality and Safety Engineering, China Jiliang University, Hangzhou 310018, China.
Zhejiang Hechuan Technology Co., Ltd., Quzhou Haichuang Park, Wenyi Road, Hangzhou 324400, China.
Micromachines (Basel). 2022 Oct 18;13(10):1764. doi: 10.3390/mi13101764.
A microfluidic impedance sensor embedded with gold interdigitated array microelectrodes was utilized to rapidly detect Tomato Ringspot Virus (ToRSV) and achieve efficient and precise detection. The electrochemical impedance spectrum was obtained by immobilizing ToRSV antibody on the surface of a gold interdigital array microelectrode and mixing it with ToRSV to generate an impedance change. The electrochemical impedance spectrum was obtained. The equivalent circuit was established to analyze the mechanism of impedance change, and the quantitative linear relationship between ToRSV concentration and impedance was established. According to an equivalent circuit analysis, ToRSV increases the solution resistance Rs, the electron transfer resistance Ret on the electrode surface, and the double layer capacitance Cdl, resulting in an increase in impedance. The results reveal that the ToRSV concentration detected in the range of 0.001 to 10 μg/mL ranges from 248.8 to 687.2 kΩ at the ideal detection frequency of 10.7 Hz, with a good linear connection, R = 0.98. When this method's detection limit is tested, the impedance value is 367.68 kΩ. 0.0032 μg/mL was the detection limit. The sensor is quick and easy to use, has high detection sensitivity, and can be used to detect other plant viruses.
一种嵌入金叉指阵列微电极的微流控阻抗传感器被用于快速检测番茄环斑病毒（ToRSV），并实现高效精确的检测。通过将ToRSV抗体固定在金叉指阵列微电极表面，并与ToRSV混合以产生阻抗变化，从而获得电化学阻抗谱。建立了等效电路来分析阻抗变化的机制，并建立了ToRSV浓度与阻抗之间的定量线性关系。根据等效电路分析，ToRSV增加了溶液电阻Rs、电极表面的电子转移电阻Ret和双层电容Cdl，导致阻抗增加。结果表明，在理想检测频率10.7 Hz下，检测浓度范围为0.001至10 μg/mL的ToRSV时，阻抗范围为248.8至687.2 kΩ，线性关系良好，R = 0.98。在测试该方法的检测限时，阻抗值为367.68 kΩ。检测限为0.0032 μg/mL。该传感器使用快速简便，具有高检测灵敏度，可用于检测其他植物病毒。
