Reddersen Kirsten, Tittelbach Jörg, Wiegand Cornelia
Klinik für Hautkrankheiten, Universitätsklinikum Jena, 07743 Jena, Germany.
Microorganisms. 2022 Oct 13;10(10):2027. doi: 10.3390/microorganisms10102027.
The treatment of chronic wounds presents a major challenge in medical care. In particular, the effective treatment of bacterial infections that occur in the form of biofilms is of crucial importance. To develop successful antibiofilm strategies for chronic wound treatment, biofilm models are needed that resemble the in vivo situation, are easy to handle, standardizable, and where results are readily transferable to the clinical situation. We established two 3D biofilm models to distinguish the effectiveness of wound dressings on important microorganisms present in chronic wounds. The first 3D biofilm model contains , , and , while the second is based on . Bacteria are cultivated in a nutrient-rich agar/gelatin mix, into which air bubbles are incorporated. This results in a mature biofilm growing in clusters similar to its organization in chronic wounds. The models are convenient to use, have low variability and are easy to establish in the laboratory. Treatment with polihexanide and silver-containing wound dressings showed that the models are very well suited for antimicrobial testing and that they can detect differences in the efficacy of antimicrobial substances. Therefore, these models present valuable tools in the development of effective antibiofilm strategies in chronic wounds.
慢性伤口的治疗是医疗护理中的一项重大挑战。特别是，有效治疗以生物膜形式出现的细菌感染至关重要。为了开发用于慢性伤口治疗的成功抗生物膜策略，需要类似于体内情况、易于操作、可标准化且结果易于转化为临床情况的生物膜模型。我们建立了两种三维生物膜模型，以区分伤口敷料对慢性伤口中存在的重要微生物的有效性。第一个三维生物膜模型包含……，而第二个基于……。细菌在富含营养的琼脂/明胶混合物中培养，并在其中掺入气泡。这导致成熟的生物膜以簇状生长，类似于其在慢性伤口中的组织形式。这些模型使用方便，变异性低，且易于在实验室中建立。用聚己缩胍和含银伤口敷料进行的治疗表明，这些模型非常适合抗菌测试，并且能够检测抗菌物质疗效的差异。因此，这些模型是开发慢性伤口有效抗生物膜策略的宝贵工具。
