National Institute for Research and Development of Optoelectronics Bucharest INOE 2000, Research Institute for Analytical Instrumentation, 67 Donath Street, 400293 Cluj-Napoca, Romania.
Molecules. 2022 Oct 11;27(20):6794. doi: 10.3390/molecules27206794.
The present study uses the Taguchi method of experimental design to optimize lipid extraction from Spirulina spp. by ultrasound application and mechanical stirring. A Taguchi L9 orthogonal array was used to optimize various parameters, such as methanol: chloroform (M:C) ratio, biomass: solvent ratio, and extraction time for lipid extraction. The results were analyzed using the signal-to-noise (S/N) ratio and analysis of variance (ANOVA). The biomass: solvent ratio significantly influenced lipid content (p < 0.05) with 92.1% and 92.3% contributions to the lipid and S/N ratio data, respectively. The extraction time presented a contribution value of 5.0%, while the M:C ratio presented the most negligible contribution of 0.4% for S/N data. The optimum extraction conditions were: M:C ratio of 1:1, biomass: solvent ratio of 1:60, and extraction time of 30 min. The predominant fatty acids were palmitic acid (44.5%), linoleic acid (14.9%), and gamma-linolenic acid (13.4%). The confirmation experiments indicated a lipid content of 8.7%, within a 95% confidence interval, proving the Taguchi method’s effectiveness in optimizing the process parameters for lipid extraction.
本研究采用田口实验设计法，通过超声应用和机械搅拌优化螺旋藻的脂质提取。采用田口 L9 正交数组优化各种参数，如甲醇：氯仿（M:C）比、生物质：溶剂比和提取时间。结果采用信噪比（S/N）比和方差分析（ANOVA）进行分析。生物质：溶剂比对脂质含量有显著影响（p < 0.05），对脂质和 S/N 比数据的贡献分别为 92.1%和 92.3%。提取时间的贡献值为 5.0%，而 M:C 比的 S/N 数据的贡献最小，为 0.4%。最佳提取条件为：M:C 比为 1:1、生物质：溶剂比为 1:60、提取时间为 30 分钟。主要脂肪酸为棕榈酸（44.5%）、亚油酸（14.9%）和γ-亚麻酸（13.4%）。验证实验表明，脂质含量为 8.7%，置信区间为 95%，证明了田口法在优化脂质提取工艺参数方面的有效性。
