Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China.
Department of Central Laboratory, The First People's Hospital of Huzhou, First Affiliated Hospital of Huzhou University, Huzhou 313000, China.
Molecules. 2022 Oct 18;27(20):6995. doi: 10.3390/molecules27206995.
Advanced glycation end products (AGEs) are the compounds produced by non-enzymatic glycation of proteins, which are involved in diabetic-related complications. To investigate the potential anti-glycation activity of Myriocin (Myr), a fungal metabolite of , the effect of Myr on the formation of AGEs resulted from the glycation of bovine serum albumin (BSA) and the interaction between Myr and BSA were studied by multiple spectroscopic techniques and computational simulations. We found that Myr inhibited the formation of AGEs at the end stage of glycation reaction and exhibited strong anti-fibrillation activity. Spectroscopic analysis revealed that Myr quenched the fluorescence of BSA in a static process, with the possible formation of a complex (approximate molar ratio of 1:1). The binding between BSA and Myr mainly depended on van der Waals interaction, hydrophobic interactions and hydrogen bond. The synchronous fluorescence and UV-visible (UV-) spectra results indicated that the conformation of BSA altered in the presence of Myr. The fluorescent probe displacement experiments and molecular docking suggested that Myr primarily bound to binding site 1 (subdomain IIA) of BSA. These findings demonstrate that Myr is a potential anti-glycation agent and provide a theoretical basis for the further functional research of Myr in the prevention and treatment of AGEs-related diseases.
糖基化终产物（AGEs）是蛋白质非酶糖化产生的化合物，与糖尿病相关并发症有关。为了研究麦角甾醇（Myr）的潜在抗糖化活性，麦角甾醇是一种真菌代谢产物，研究了麦角甾醇对牛血清白蛋白（BSA）糖化生成 AGEs 的形成的影响，以及麦角甾醇与 BSA 之间的相互作用，采用了多种光谱技术和计算模拟。我们发现，麦角甾醇可以在糖化反应的终末阶段抑制 AGEs 的形成，并具有很强的抗纤维化活性。光谱分析表明，麦角甾醇以静态过程猝灭 BSA 的荧光，可能形成复合物（约摩尔比为 1:1）。BSA 与麦角甾醇之间的结合主要取决于范德华相互作用、疏水相互作用和氢键。同步荧光和紫外可见（UV-）光谱结果表明，麦角甾醇存在时 BSA 的构象发生了改变。荧光探针置换实验和分子对接表明，麦角甾醇主要结合到 BSA 的结合位点 1（亚域 IIA）。这些发现表明麦角甾醇是一种潜在的抗糖化剂，为进一步研究麦角甾醇在预防和治疗 AGEs 相关疾病中的功能提供了理论依据。
