School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
School of Chemistry and Chemical Engineering, Xi'an University of Science and Technology, Xi'an 710054, China.
Molecules. 2022 Oct 21;27(20):7140. doi: 10.3390/molecules27207140.
(PE) is an edible mushroom with high nutritional value. polysaccharides (PEPs) are one of the main active ingredients and manifest a great variety of biological activities. This study mainly focused on the chemical characterization and biological activities of PEPs, which were separated into two fractions (named WPS and P-1). WPS is mainly dominated by -glycosidic bonds and contains -glycosidic bonds, and P-1 only contains -glycosidic bonds. The molecular weights of WPS and P-1 were 4.5 × 10 Da and 2.2 × 10 Da. The result of GC indicated that two the fractions were composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose, with a ratio of 0.35:0.24:0.45:0.24:28.78:1.10 for WPS and 0.95:0.64:0.66:1.84:60.69:0.67 for P-1. The advanced structure studies indicated that the two fractions had no triple-helical structure, where WPS had a dense structure and P-1 had a loose structure. In addition, the antioxidant activity of WPS surpassed P-1, and the two fractions also exhibited a high hypoglycemic activity via inhibiting -glycosidase activities and promoting the expression of PI3K-AKT signaling pathway based on in vitro assay and cell experiments.
(PE) 是一种具有高营养价值的食用蘑菇。多糖 (PEPs) 是其主要活性成分之一，表现出多种生物活性。本研究主要集中于 PEPs 的化学特性和生物活性，将其分离为两个部分（命名为 WPS 和 P-1）。WPS 主要由 -糖苷键组成，含有 -糖苷键，而 P-1 仅含有 -糖苷键。WPS 和 P-1 的分子量分别为 4.5×10 Da 和 2.2×10 Da。GC 结果表明，这两个部分由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖组成，其比例为 WPS 为 0.35:0.24:0.45:0.24:28.78:1.10，P-1 为 0.95:0.64:0.66:1.84:60.69:0.67。高级结构研究表明，这两个部分没有三螺旋结构，其中 WPS 具有致密结构，而 P-1 具有松散结构。此外，WPS 的抗氧化活性超过了 P-1，这两个部分还通过抑制 -糖苷酶活性和促进 PI3K-AKT 信号通路的表达，表现出很高的降血糖活性，这是基于体外测定和细胞实验得出的结果。
