Department of Sciences, University of Basilicata, 85100 Potenza, Italy.
Department of Biosciences, Biotechnologies and Environment, University of Bari "Aldo Moro", 70126 Bari, Italy.
Molecules. 2023 Feb 26;28(5):2176. doi: 10.3390/molecules28052176.
In this study the antioxidant and neuroprotective activity of an enriched polysaccharide fraction (EPF) obtained from the fruiting body of cultivated was evaluated. Proximate composition (moisture, proteins, fat, carbohydrates and ash) was determined using the AOAC procedures. The EPF was extracted by using, in sequence, hot water and alkaline extractions followed by deproteinization and precipitation with cold ethanol. Total α- and β-glucans were quantified using the Megazyme International Kit. The results showed that this procedure allows a high yield of polysaccharides with a higher content of (1-3; 1-6)-β-D-glucans. The antioxidant activity of EPF was detected from the total reducing power, DPPH, superoxide, hydroxyl and nitric oxide radical scavenging activities. The EPF was found to scavenge DPPH, superoxide, hydroxyl and nitric oxide radicals with a IC50 values of 0.52 ± 0.02, 1.15 ± 0.09, 0.89 ± 0.04 and 2.83 ± 0.16 mg/mL, respectively. As assessed by the MTT assay, the EPF was biocompatible for DI-TNC1 cells in the range of 0.006-1 mg/mL and, at concentrations ranging from 0.05 to 0.2 mg/mL, significantly counteracted HO-induced reactive oxygen species production. This study demonstrated that polysaccharides extracted from might be used as functional food to potentiate the antioxidant defenses and to reduce oxidative stress.
在这项研究中，评估了从栽培 子实体中获得的富含多糖部分 (EPF) 的抗氧化和神经保护活性。使用 AOAC 程序测定了近似成分（水分、蛋白质、脂肪、碳水化合物和灰分）。EPF 通过依次使用热水和碱性提取，然后用冷乙醇进行脱蛋白和沉淀来提取。使用 Megazyme International Kit 定量了总 α-和 β-葡聚糖。结果表明，该方法允许获得具有更高（1-3；1-6）-β-D-葡聚糖含量的多糖的高产率。通过总还原力、DPPH、超氧化物、羟基和一氧化氮自由基清除活性检测了 EPF 的抗氧化活性。结果表明，EPF 对 DPPH、超氧化物、羟基和一氧化氮自由基具有清除作用，其 IC50 值分别为 0.52 ± 0.02、1.15 ± 0.09、0.89 ± 0.04 和 2.83 ± 0.16 mg/mL。通过 MTT 测定评估，EPF 在 0.006-1 mg/mL 范围内对 DI-TNC1 细胞具有生物相容性，并且在 0.05-0.2 mg/mL 浓度范围内，显著抑制了 HO 诱导的活性氧物质产生。这项研究表明，从 中提取的多糖可用作功能性食品，以增强抗氧化防御并减少氧化应激。
