Palierse Estelle, Masse Sylvie, Laurent Guillaume, Le Griel Patrick, Mosser Gervaise, Coradin Thibaud, Jolivalt Claude
Sorbonne Université, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, 75005 Paris, France.
Sorbonne Université, CNRS, Laboratoire de Réactivité de Surface, 75005 Paris, France.
Nanomaterials (Basel). 2022 Oct 13;12(20):3588. doi: 10.3390/nano12203588.
Plant-derived natural bioactive molecules are of great therapeutic potential but, so far, their application in nanomedicine has scarcely been studied. This work aimed at comparing two methodologies, i.e., adsorption and in situ incorporation, to prepare hybrid polyphenol/hydroxyapatite nanoparticles. Two flavonoids, baicalin and its aglycone derivative baicalein, and two phenolic acids derived from caffeic acid, rosmarinic and chlorogenic acids, were studied. Adsorption of these polyphenols on pre-formed hydroxyapatite nanoparticles did not modify particle size or shape and loading was less than 10% (/). In contrast, presence of polyphenols during the synthesis of nanoparticles significantly impacted and sometimes fully inhibited hydroxyapatite formation but recovered particles could exhibit higher loadings. For most hybrid particles, release profiles consisted of a 24 h burst effect followed by a slow release over 2 weeks. Antioxidant properties of the polyphenols were preserved after adsorption but not when incorporated in situ. These results provide fruitful clues for the valorization of natural bioactive molecules in nanomedicine.
植物源天然生物活性分子具有巨大的治疗潜力，但迄今为止，它们在纳米医学中的应用鲜有研究。这项工作旨在比较两种制备多酚/羟基磷灰石杂化纳米颗粒的方法，即吸附法和原位掺入法。研究了两种黄酮类化合物黄芩苷及其苷元衍生物黄芩素，以及两种源自咖啡酸的酚酸，迷迭香酸和绿原酸。这些多酚在预先形成的羟基磷灰石纳米颗粒上的吸附并未改变颗粒大小或形状，负载量小于10%（/）。相反，在纳米颗粒合成过程中多酚的存在显著影响，有时甚至完全抑制羟基磷灰石的形成，但回收的颗粒可能表现出更高的负载量。对于大多数杂化颗粒，释放曲线包括24小时的突释效应，随后在2周内缓慢释放。多酚的抗氧化性能在吸附后得以保留，但原位掺入时则不然。这些结果为天然生物活性分子在纳米医学中的应用提供了有益线索。
