Efthimiou Ioanna, Vlastos Dimitris, Triantafyllidis Vassilios, Eleftherianos Antonios, Antonopoulou Maria
Department of Sustainable Agriculture, University of Patras, GR-30100 Agrinio, Greece.
Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research (HCMR), GR-19013 Anavyssos, Greece.
Plants (Basel). 2022 Oct 11;11(20):2673. doi: 10.3390/plants11202673.
belongs to the family and is most common in the northern hemisphere. Various birch species have exhibited antimicrobial, antioxidant and anticancer properties. In the present study, we investigated the genotoxic and cytotoxic activity as well as the antigenotoxic potential against the mutagenic agent mitomycin-C (MMC) of two commercial products, i.e., a aqueous leaf extract product (BE) and a product containing aqueous extract of birch leaves at a percentage of 94% and lemon juice at a percentage of 6% (BP) using the cytokinesis block micronucleus (CBMN) assay. The most prevalent compounds and elements of BE and BP were identified using UHPLC-MS and ICP-MS/MS, respectively. All mixtures of BE with MMC demonstrated a decrease in the MN frequencies, with the lowest and highest concentrations inducing a statistically significant antigenotoxic activity. BP lacked genotoxic potential, while it was cytotoxic in all concentrations. Its mixtures with MMC demonstrated statistically significant antigenotoxic activity only at the lowest concentration. UHPLC-MS and ICP-MS/MS showed the presence of various elements and phytochemicals. Our results reveal antigenotoxic and cytotoxic potential of both BE and BP, while the variations observed could indicate the importance of the interactions among different natural products and/or their compounds.
属于该科，在北半球最为常见。各种桦树品种已表现出抗菌、抗氧化和抗癌特性。在本研究中，我们使用胞质分裂阻滞微核（CBMN）试验，研究了两种商业产品的遗传毒性和细胞毒性活性以及对诱变剂丝裂霉素-C（MMC）的抗诱变潜力，即一种桦树叶水提取物产品（BE）和一种含有94%桦树叶水提取物和6%柠檬汁的产品（BP）。分别使用超高效液相色谱-质谱联用仪（UHPLC-MS）和电感耦合等离子体质谱/质谱联用仪（ICP-MS/MS）鉴定了BE和BP中最常见的化合物和元素。BE与MMC的所有混合物均显示微核频率降低，最低和最高浓度诱导出具有统计学意义的抗诱变活性。BP缺乏遗传毒性潜力，但其在所有浓度下均具有细胞毒性。其与MMC的混合物仅在最低浓度下显示出具有统计学意义的抗诱变活性。UHPLC-MS和ICP-MS/MS显示存在各种元素和植物化学物质。我们的结果揭示了BE和BP的抗诱变和细胞毒性潜力，而观察到的差异可能表明不同天然产物和/或其化合物之间相互作用的重要性。
