Raal Ain, Kaldmäe Hedi, Kütt Karin, Jürimaa Katrin, Silm Maidu, Bleive Uko, Aluvee Alar, Adamson Kalev, Vester Marili, Erik Mart, Koshovyi Oleh, Nguyen Khan Viet, Nguyen Hoai Thi, Drenkhan Rein
Institute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, Estonia.
Polli Horticultural Research Centre, Chair of Horticulture, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Uus 2, Polli, 69108 Mulgi Parish, Estonia.
Pharmaceuticals (Basel). 2024 Aug 1;17(8):1013. doi: 10.3390/ph17081013.
Chaga mushroom () is a pathogenic fungus that grows mostly on birch species ( Roth and Ehrh.) and has traditionally been used as an anticancer medicine. This study aimed to compare the chemical composition and cytotoxic activity of chagas growing on both spp. on various cancer cell lines. The freeze-dried extracts contained triterpenes inotodiol, lanosterol betulin, and betulinic acid typical to conks growing on species. The cytotoxic activity of chaga growing on and 80% ethanolic extracts against 31 human cancer cell lines was evaluated by a sulforhodamine B assay. Chaga extract showed moderate activity against all cancer cell lines examined; it did not result in high cytotoxicity (IC ≤ 20 µg/mL). The strongest inhibitions were observed with chaga (growing on ) extract on the HepG2 and CAL-62 cell line and with chaga (from ) extract on the HepG2 cell line, with IC values of 37.71, 43.30, and 49.99 μg/mL, respectively. The chaga extracts from exert somewhat stronger effects on most cancer cell lines studied than extracts, which can be attributed to a higher content of inotodiol in extracts. This study highlights the potential of chaga as a source of bioactive compounds with selective anticancer properties. To the best of our knowledge, this study is the first investigation of the chemical composition of parasitizing on .
桦褐孔菌是一种主要生长在桦木属植物（Roth和Ehrh.）上的致病真菌，传统上被用作抗癌药物。本研究旨在比较生长在不同桦木属植物上的桦褐孔菌的化学成分和细胞毒性活性，以及它们对各种癌细胞系的影响。冻干提取物含有三萜类化合物氧化硬脂醇、羊毛甾醇、桦木醇和桦木酸，这些是生长在桦木属植物上的子实体所特有的。通过磺基罗丹明B测定法评估了生长在两种桦木属植物上的桦褐孔菌80%乙醇提取物对31种人类癌细胞系的细胞毒性活性。桦褐孔菌提取物对所有检测的癌细胞系均表现出中等活性；未导致高细胞毒性（IC≤20μg/mL）。观察到生长在某种桦木属植物上的桦褐孔菌提取物对HepG2和CAL-62细胞系的抑制作用最强，而来自另一种桦木属植物的桦褐孔菌提取物对HepG2细胞系的抑制作用最强，IC值分别为37.71、43.30和49.99μg/mL。与另一种提取物相比，来自某种桦木属植物的桦褐孔菌提取物对大多数研究的癌细胞系的作用稍强，这可能归因于该提取物中氧化硬脂醇的含量较高。本研究突出了桦褐孔菌作为具有选择性抗癌特性的生物活性化合物来源的潜力。据我们所知，本研究是首次对寄生在桦木属植物上的桦褐孔菌的化学成分进行研究。
