Francis Dali Vilma, Sood Neeru, Gokhale Trupti
Department of Biotechnology, Birla Institute of Technology and Science, Dubai Campus, Dubai International Academic City, Dubai P.O. Box 345055, United Arab Emirates.
Plants (Basel). 2022 Oct 19;11(20):2776. doi: 10.3390/plants11202776.
The biogenic synthesis of CuO and ZnO nanoparticles (NPs) was carried out by The shape, size, and chemical identity of the CuO and ZnO NPs were determined using FTIR, XRD, SEM, EDX, and TEM analysis. The study aimed to investigate the effects of the CuO and ZnO NPs on seed germination and plant growth. Two different fertilizer application modes (hydroponics and foliar) were studied with varying concentrations of CuO (0.06 µM, 0.12 µM) and ZnO (0.12 µM, 0.24 µM) nanoparticles with water control and Hoagland's media control. The hydroponic system of fertilizer application demonstrated better efficiency in terms of plant growth as compared to the foliar application. The agronomic traits, SPAD value, total reducing sugars, antioxidant activity, amount of copper, and zinc ions in root and shoot were analyzed for all experimental plants and found better with the nanoparticle application. The highlight of the study is the application of extremely low concentrations of CuO and ZnO nanoparticles, almost 70% lower than the copper and zinc salts in the Hoagland's medium for improved plant growth. The use of lower concentrations of nanoparticles can prevent their accumulation in the environment and also lower the production cost. The high antioxidant concentration exhibited by the plants treated with CuO and ZnO nanoparticles ensures the enhanced plant's resistance to infections and pests while promoting plant growth.
通过生物合成法制备了氧化铜（CuO）和氧化锌（ZnO）纳米颗粒（NPs）。利用傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）、能谱分析（EDX）和透射电子显微镜（TEM）分析确定了CuO和ZnO NPs的形状、大小和化学特性。该研究旨在探究CuO和ZnO NPs对种子萌发和植物生长的影响。研究了两种不同的施肥方式（水培和叶面喷施），采用不同浓度的CuO（0.06 μM、0.12 μM）和ZnO（0.12 μM、0.24 μM）纳米颗粒，并设置了水对照和霍格兰氏培养基对照。与叶面喷施相比，水培施肥系统在植物生长方面表现出更高的效率。对所有实验植物的农艺性状、SPAD值、总还原糖、抗氧化活性、根和茎中铜和锌离子的含量进行了分析，发现施用纳米颗粒后效果更好。该研究的亮点是应用极低浓度的CuO和ZnO纳米颗粒，其浓度比霍格兰氏培养基中的铜盐和锌盐低近70%，却能促进植物生长。使用较低浓度的纳米颗粒可以防止它们在环境中积累，同时降低生产成本。用CuO和ZnO纳米颗粒处理的植物表现出高抗氧化剂浓度，这确保了植物对病虫害的抵抗力增强，同时促进植物生长。
