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人多形核细胞(PMN)对32P标记大肠杆菌的吞噬作用。一种方法的改进

Phagocytosis of 32P-labelled E. coli by human polymorphonuclear cells (PMN). Adaptation of a method.

作者信息

Midtvedt T, Melby K

出版信息

Acta Pathol Microbiol Scand B. 1978 Aug;86(4):193-9. doi: 10.1111/j.1699-0463.1978.tb00031.x.

Abstract

A system for the study of phagocytosis by human polymorphonuclear cells (PMN) is presented. The leucocytes are harvested from heparinized whole blood by the Bøyum method and transferred to glass tubes to yield glass adherent monolayers of leucocytes, approximately 80% of which were PMN. A strain of E. coli labelled by 32P served as test organism.

摘要

本文介绍了一种用于研究人多形核细胞(PMN)吞噬作用的系统。通过博伊姆法从肝素化全血中采集白细胞,并转移至玻璃管中,以形成白细胞的玻璃贴壁单层,其中约80%为PMN。用32P标记的大肠杆菌菌株作为测试生物。

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