利用 DNA 条码标记对野生和栽培草莓（ × ）进行分子特征分析。

Molecular Characterization of Wild and Cultivated Strawberry ( × ) through DNA Barcode Markers.

机构信息

Department of Genetics, Hazara University Mansehra, Mansehra, Pakistan.

Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China.

出版信息

Genet Res (Camb). 2022 Oct 11;2022:9249561. doi: 10.1155/2022/9249561. eCollection 2022.

DOI:10.1155/2022/9249561

PMID:36299683

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9578897/

Abstract

BACKGROUND

DNA barcoding is a useful technique for the identification, conservation, and diversity estimation at the species level in plants. The current research work was carried out to characterize selected species from northern Pakistan using DNA barcode markers. . Initially, the efficacy of eight DNA barcode markers was analyzed based on the amplification and sequencing of the genome of selected species. The resultant sequences were analyzed using BLAST, MEGA 7.0, and Bio Edit software. The phylogenetic tree was constructed by using current species sequences and reference sequences through the neighbor-joining method or maximum likelihood method.

RESULTS

Among eight DNA barcode markers, only two ( and ) were amplified, and sequences were obtained. sequence was BLAST in NCBI for related reference species which ranged from 89.79% to 90.05% along with (AF163517.1) which have 99.05% identity. Similarly, the sequence of species was ranged from 96% to 99.58% along with × (KY358226.1) which had 99.58% identity.

CONCLUSION

It is recommended that DNA barcode markers are a useful tool to identify the genetic diversity of a species. Moreover, this study could be helpful for the identification of the species cultivated in other regions of the world.

摘要

背景

DNA 条形码技术是一种在植物物种水平上进行鉴定、保护和多样性估计的有用技术。本研究旨在利用 DNA 条形码标记物对来自巴基斯坦北部的选定物种进行特征描述。最初，根据选定物种基因组的扩增和测序分析了 8 个 DNA 条形码标记的功效。使用 BLAST、MEGA 7.0 和 Bio Edit 软件分析所得序列。通过邻接法或最大似然法，利用当前物种序列和参考序列构建系统发育树。

结果

在所研究的 8 个 DNA 条形码标记物中，仅扩增并获得了两个标记物（和）的序列。在 NCBI 中，对序列进行了 BLAST 同源性检索，与参考物种的同源性范围为 89.79%至 90.05%，与（AF163517.1）的同源性为 99.05%。同样，对物种的序列进行了 BLAST 同源性检索，与参考物种的同源性范围为 96%至 99.58%，与 × （KY358226.1）的同源性为 99.58%。