Pang Xiaohui, Song Jingyuan, Zhu Yingjie, Xu Hongxi, Huang Linfang, Chen Shilin
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, PR China.
Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Hong Kong, PR China.
Cladistics. 2011 Apr;27(2):165-170. doi: 10.1111/j.1096-0031.2010.00328.x.
© The Willi Hennig Society 2010. ABSTRACT: The Consortium for the Barcode of Life (CBOL) Plant Working Group (PWG) established the use of matK+rbcL as core barcodes and ITS2 as one of the supplementary loci for differentiating plants at the Third International Barcoding Conference. Here, we tested the applicability of four DNA regions (rbcL, matK, rpoC1 and ITS2) as the barcodes for identifying species within Rosaceae. Based on assessments of the success rates of PCR amplifications, the sequence quality, extent of specific genetic divergence, DNA barcoding gap and ability for species discrimination, our results suggest that ITS2 is the best of the four loci tested for barcoding Rosaceae. We further evaluated the effectiveness of ITS2 for identifying a wide range of species within Rosaceae. Of the 1410 plant samples collected from 893 species in 96 diverse genera, ITS2 successfully identified 78 and 100% of them at the species and genus levels, respectively. Therefore, our research indicated that the ITS2 region is a powerful, though not perfect, barcode for Rosaceae identification that also contributes valuable information for identifying closely related species in other plant taxonomic groups.
©威利·亨尼希协会2010年。摘要：生命条形码联盟（CBOL）植物工作组（PWG）在第三届国际条形码会议上确定使用matK+rbcL作为核心条形码，ITS2作为区分植物的补充位点之一。在此，我们测试了四个DNA区域（rbcL、matK、rpoC1和ITS2）作为蔷薇科物种鉴定条形码的适用性。基于对PCR扩增成功率、序列质量、特定遗传分化程度、DNA条形码间隙和物种鉴别能力的评估，我们的结果表明，ITS2是所测试的四个蔷薇科条形码位点中最好的。我们进一步评估了ITS2在鉴定蔷薇科广泛物种方面的有效性。在从96个不同属的893个物种中收集的1410个植物样本中，ITS2在物种和属水平上分别成功鉴定了其中的78%和100%。因此，我们的研究表明，ITS2区域是用于蔷薇科鉴定的一个强大但并非完美的条形码，它也为鉴定其他植物分类群中的近缘物种提供了有价值的信息。
