Xiao Luning, Jin Yuli, Liu Wei, Liu Jie, Song Huajie, Li Dong, Zheng Jianpeng, Wang Dongmei, Yin Yan, Liu Yang, Wang Hao, Li Linzhi, Sun Nina, Liu Minxiao, Ma Pengtao
College of Life Sciences, Yantai University, Yantai, China.
Institute of Grain and Oil Crops, Yantai Academy of Agricultural Sciences, Yantai, China.
Front Plant Sci. 2022 Oct 10;13:1037027. doi: 10.3389/fpls.2022.1037027. eCollection 2022.
Fungal diseases, drought, pre-harvest sprouting (PHS) and other biotic and abiotic stresses have seriously affected the quality and yield in wheat production. Identifying related genes/loci in released cultivars/lines can provide reference information and theoretical basis for wheat improvement. Yannong series wheat cultivars/lines have distinctive characteristics in wheat cultivars and play an important role in genetic improvement and production of Chinese wheat production system. To dissect their genetic basis of the stress-resistant traits, in this study, 23 representative Yannong series wheat cultivars/lines were tested by 58 molecular markers for 40 genes related to adaptability, disease resistance and stress tolerance to clarify the genetic composition of the key loci. The results showed that most of the tested wheat accessions carried dwarfing genes // and recessive vernalization genes ///. It was also consistent with the phenotypic traits of tested Yannong series wheat which were dwarf and winter or semi winter wheat. In addition, the overall level of seedling powdery mildew resistance in 23 Yannong wheat cultivars/lines was moderate or inadequate. Eleven accessions carried none of the tested genes and twelve accessions carried , , and singly or in combination. Then, 23 wheat cultivars/lines were also tested by 17 diagnostic markers for 14 genes. The results showed that 16 wheat cultivars/lines were likely to carry one or more of tested genes, whereas Yannong 15, Yannong 17, Yannong 23, Yannong 24, Yannong 377, Yannong 572 and Yannong 999 carried none of the tested genes. Moreover, in our study, nine markers for four genes related to drought tolerance and PHS were used to evaluate the stress tolerance of the 23 wheat cultivars/lines. The results indicated that all 23 wheat cultivars/lines carried drought resistance genes , indicating that they had the drought resistance to the extent. Except for Yannong 30, Yannong 377, Yannong 390, Yannong 745 and Yannong 1766, other wheat cultivars/lines carried one to three elite PHS-resistant alleles .
真菌病害、干旱、穗发芽（PHS）以及其他生物和非生物胁迫严重影响了小麦生产的品质和产量。在已发布的品种/品系中鉴定相关基因/位点可为小麦改良提供参考信息和理论依据。烟农系列小麦品种/品系在小麦品种中具有独特特征，在中国小麦生产体系的遗传改良和生产中发挥着重要作用。为剖析其抗逆性状的遗传基础，本研究利用58个分子标记对23个具有代表性的烟农系列小麦品种/品系进行检测，这些标记与40个与适应性、抗病性和抗逆性相关的基因有关，以阐明关键位点的遗传组成。结果表明，大多数被测小麦材料携带矮秆基因//和隐性春化基因///。这也与被测烟农系列小麦的表型性状一致，即矮秆且为冬性或半冬性小麦。此外，23个烟农小麦品种/品系的苗期白粉病抗性总体水平中等或不足。11个材料未携带任何被测基因，12个材料单独或组合携带、、和。然后，还利用17个针对14个基因的诊断标记对23个小麦品种/品系进行检测。结果表明，16个小麦品种/品系可能携带一个或多个被测基因，而烟农15、烟农17、烟农23、烟农24、烟农377、烟农572和烟农999未携带任何被测基因。此外，在本研究中，利用与耐旱性和穗发芽相关的4个基因的9个标记对23个小麦品种/品系的抗逆性进行评估。结果表明，所有23个小麦品种/品系均携带抗旱基因，表明它们在一定程度上具有抗旱性。除烟农30、烟农377、烟农390、烟农745和烟农1766外，其他小麦品种/品系携带1至3个优良的抗穗发芽等位基因。
