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挖掘小麦等位基因用于目标导向的标记辅助育种。

Mining of Wheat Alleles for Goal-Oriented Marker-Assisted Breeding.

作者信息

Yu Ziyang, Xiao Luning, Su Fuyu, Liu Wei, Luo Fuyi, Han Ran, Mu Yanjun, Zhang Wenjing, Wu Liru, Liang Xiao, Sun Nina, Li Linzhi, Ma Pengtao

机构信息

College of Life Sciences, Yantai University, Yantai, China.

Institute of Grain and Oil Crops, Yantai Academy of Agricultural Sciences, Yantai, China.

出版信息

Front Plant Sci. 2022 May 12;13:912589. doi: 10.3389/fpls.2022.912589. eCollection 2022.

DOI:10.3389/fpls.2022.912589
PMID:35646019
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9133932/
Abstract

Powdery mildew of wheat, caused by f. sp. (), is a devastating disease that seriously reduces yield and quality worldwide. Utilization of plant resistance genes is an attractive and effective strategy for controlling this disease. Among the reported powdery mildew () resistance genes, exhibits a diverse resistance spectrum among its multiple alleles. It has been widely used in China for resistance breeding for powdery mildew. To mine more alleles and clarify their distribution, we screened 33 wheat cultivars/breeding lines carrying alleles from 641 wheat genotypes using diagnostic and -linked markers. To further investigate the relationships within the alleles, we compared their resistance spectra, polymorphism of marker alleles and gene sequences, and found that they have identical marker alleles and gene sequences but diverse resistance spectra. In addition, the diagnostic kompetitive allele-specific PCR (KASP) marker, , was developed and was shown to detect all the alleles in the different genetic backgrounds. These findings provide valuable information for the distribution and rational use of alleles, push forward their marker-assisted breeding (MAS), and hence improve the control of wheat powdery mildew.

摘要

由小麦白粉菌（Blumeria graminis f. sp. tritici）引起的小麦白粉病是一种毁灭性病害，在全球范围内严重降低产量和品质。利用植物抗性基因是防治该病害的一种有吸引力且有效的策略。在已报道的小麦白粉病抗性基因中，Pm21在其多个等位基因中表现出多样的抗性谱。它在中国已被广泛用于小麦白粉病抗性育种。为挖掘更多Pm21等位基因并阐明其分布情况，我们使用诊断性标记和与Pm21连锁的标记，从641个小麦基因型中筛选出33个携带Pm21等位基因的小麦品种/育种系。为进一步研究Pm21等位基因之间的关系，我们比较了它们的抗性谱、标记等位基因多态性和基因序列，发现它们具有相同的标记等位基因和基因序列，但抗性谱不同。此外，还开发了诊断性 kompetitive等位基因特异性PCR（KASP）标记Xmag4448，并证明其能检测不同遗传背景下的所有Pm21等位基因。这些发现为Pm21等位基因的分布和合理利用提供了有价值的信息，推动了其分子标记辅助育种（MAS），从而有助于更好地防治小麦白粉病。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/6204bd334245/fpls-13-912589-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/6f3c2226b357/fpls-13-912589-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/b9d9dacc8b4e/fpls-13-912589-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/df9b38832151/fpls-13-912589-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/8f3d542523f2/fpls-13-912589-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/79f49f321808/fpls-13-912589-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/5b5edb8e4db7/fpls-13-912589-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/6204bd334245/fpls-13-912589-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/6f3c2226b357/fpls-13-912589-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/b9d9dacc8b4e/fpls-13-912589-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/df9b38832151/fpls-13-912589-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/8f3d542523f2/fpls-13-912589-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/79f49f321808/fpls-13-912589-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/5b5edb8e4db7/fpls-13-912589-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc24/9133932/6204bd334245/fpls-13-912589-g007.jpg

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