Mu Yanjun, Gong Wenping, Qie Yanmin, Liu Xueqing, Li Linzhi, Sun Nina, Liu Wei, Guo Jun, Han Ran, Yu Ziyang, Xiao Luning, Su Fuyu, Zhang Wenjing, Wang Jiangchun, Han Guohao, Ma Pengtao
College of Life Sciences, Yantai University, Yantai, China.
Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, China.
Front Plant Sci. 2022 Sep 6;13:1005627. doi: 10.3389/fpls.2022.1005627. eCollection 2022.
Powdery mildew of wheat (), caused by f.sp. (), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13-205 (JH13-205). The result indicated that a single recessive gene, tentatively designated , conferred seedling resistance to the isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), was physically located to a ~33.7 Mb (691.0-724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2-708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, differed from those of the reported powdery mildew () resistance genes on 2BL, suggesting is most likely a new gene/allele in the targeted interval. To transfer to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for can be used in MAS for tracking when it transferred into those susceptible cultivars.
小麦白粉病（由小麦白粉菌引起）是一种毁灭性病害，严重威胁其寄主的产量和品质。鉴定抗性基因是培育抗病品种和防治该病害最具吸引力且有效的策略。在本研究中，小麦育种系烟农99102 - 06188（YN99102），它是与著名小麦品种烟农999处于同一育种过程的优良衍生系，在整个生育期对白粉病表现出高抗性。利用分离菌E09以及YN99102与感病亲本金禾13 - 205（JH13 - 205）杂交的群体进行遗传分析。结果表明，一个单隐性基因（暂命名为）赋予对分离菌E09的苗期抗性。通过混合分离群体外显子捕获测序（BSE - Seq），将定位到2BL染色体臂上约33.7 Mb（691.0 - 724.7 Mb）的区间，并且利用分子标记将该区间进一步锁定在1.5 cM的遗传区间，该遗传区间对应9.0 Mb的物理区间（699.2 - 708.2 Mb）。基于物理定位、起源、抗性谱和遗传模式的分析，与2BL上已报道的小麦白粉病抗性基因不同，表明在目标区间很可能是一个新的抗性基因/等位基因。为了通过标记辅助选择（MAS）将转移到不同的遗传背景中，对18个紧密连锁标记在不同遗传背景下用于MAS的可用性进行了测试，当转移到那些感病品种中时，除之外的所有标记都可用于MAS追踪。
